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. 2021 Aug;58(8):3729-3744.
doi: 10.1007/s12035-021-02378-x. Epub 2021 Apr 5.

Transcription Factor VAX1 Regulates the Regional Specification of the Subpallium Through Repressing Gsx2

Yan Wen #  1 , Zihao Su #  1 , Ziwu Wang #  1 , Lin Yang  1 , Guoping Liu  1 , Zicong Shang  1 , Yangyang Duan  1 , Heng Du  1 , Zhenmeiyu Li  1 , Yan You  1 , Xiaosu Li  1 , Zhengang Yang  1 , Zhuangzhi Zhang  2
Affiliations

Transcription Factor VAX1 Regulates the Regional Specification of the Subpallium Through Repressing Gsx2

Yan Wen et al. Mol Neurobiol. 2021 Aug.

Abstract

Specification of the progenitors' regional identity is a pivotal step during development of the cerebral cortex and basal ganglia. The molecular mechanisms underlying progenitor regionalization, however, are poorly understood. Here we showed that the transcription factor Vax1 was highly expressed in the developing subpallium. In its absence, the RNA-Seq analysis, in situ RNA hybridization, and immunofluorescence staining results showed that the cell proliferation was increased in the subpallium, but the neuronal differentiation was blocked. Moreover, the dLGE expands ventrally, and the vLGE, MGE, and septum get smaller. Finally, overexpressed VAX1 in the LGE progenitors strongly inhibits Gsx2 expression. Taken together, our findings show that Vax1 is crucial for subpallium regionalization by repressing Gsx2.

Keywords: Development; Gsx2; Subpallium; Telencephalon; Vax1.

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