A rotating bottle culture method with continuous replacement of the gas phase
- PMID: 33822
- DOI: 10.1007/BF01917926
A rotating bottle culture method with continuous replacement of the gas phase
Abstract
A 'rotator' culture method is described which provides a continuous flow of oxygenating gas to cultures in rotating bottles. The system maintains constant O2 and CO2 levels in the culture medium throughout the incubation period. It also provides a more stable pH than systems with sealed culture bottles.
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