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. 1979 Jan 15;35(1):138-40.
doi: 10.1007/BF01917926.

A rotating bottle culture method with continuous replacement of the gas phase

A rotating bottle culture method with continuous replacement of the gas phase

D A New et al. Experientia. .

Abstract

A 'rotator' culture method is described which provides a continuous flow of oxygenating gas to cultures in rotating bottles. The system maintains constant O2 and CO2 levels in the culture medium throughout the incubation period. It also provides a more stable pH than systems with sealed culture bottles.

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