Concentrating Pooled COVID-19 Patient Lysates to Improve Reverse Transcription Quantitative PCR Sensitivity and Efficiency

doi:10.1093/clinchem/hvab035

. 2021 Apr 29;67(5):797-798.

doi: 10.1093/clinchem/hvab035.

Concentrating Pooled COVID-19 Patient Lysates to Improve Reverse Transcription Quantitative PCR Sensitivity and Efficiency

Harshmeena R Sanghani¹, Dorota A Nawrot², Francisco Marmolejo-Cossío³, John M Taylor⁴, Judith Craft⁴, Elizabeth Kalimeris⁵, Monique I Andersson^{5

6}, Sridhar R Vasudevan^{1

2}

Affiliations

¹ Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, UK.
² Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, UK.
³ Department of Computer Science, University of Oxford, Parks Road, Oxford, UK.
⁴ Oxford Regional Genetics Laboratory, Oxford University Hospitals NHS Foundation Trust, Oxford, UK.
⁵ Department of Microbiology, Oxford University NHS Foundation Trust, Oxford, UK.
⁶ Nuffield Division of Clinical Laboratory Science, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.

PMID: 33822907
PMCID: PMC8083470
DOI: 10.1093/clinchem/hvab035

Concentrating Pooled COVID-19 Patient Lysates to Improve Reverse Transcription Quantitative PCR Sensitivity and Efficiency

Harshmeena R Sanghani et al. Clin Chem. 2021.

. 2021 Apr 29;67(5):797-798.

doi: 10.1093/clinchem/hvab035.

Authors

Harshmeena R Sanghani¹, Dorota A Nawrot², Francisco Marmolejo-Cossío³, John M Taylor⁴, Judith Craft⁴, Elizabeth Kalimeris⁵, Monique I Andersson^{5

6}, Sridhar R Vasudevan^{1

2}

Affiliations

¹ Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, UK.
² Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, UK.
³ Department of Computer Science, University of Oxford, Parks Road, Oxford, UK.
⁴ Oxford Regional Genetics Laboratory, Oxford University Hospitals NHS Foundation Trust, Oxford, UK.
⁵ Department of Microbiology, Oxford University NHS Foundation Trust, Oxford, UK.
⁶ Nuffield Division of Clinical Laboratory Science, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.

PMID: 33822907
PMCID: PMC8083470
DOI: 10.1093/clinchem/hvab035

No abstract available

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Figures

**Fig. 1.**
A spectrum of COVID-19 pooled patient samples with and without subsequent concentration using 100 000 MWCO filters (340 samples, 34 pooled lysates).

See this image and copyright information in PMC

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References

1. Dorfman R. The detection of defective members of large populations. Ann Math Statist 1943;14:436–40.
1. Bilder CR, Tebbs JM. Pooled-testing procedures for screening high volume clinical specimens in heterogeneous populations. Statist Med 2012;31:3261–8. - PMC - PubMed
1. Erman M. What is pooled testing for COVID-19 and how can it help fight the virus? https://www.reuters.com/article/us-health-coronavirus-testing-explainer-... (Accessed August 2020).
1. Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, et al. A new coronavirus associated with human respiratory disease in China. Nature 2020;579:265–9. - PMC - PubMed
1. Pilcher CD, Fiscus SA, Nguyen TQ, Foust E, Wolf L, Williams D, et al. Detection of acute infections during HIV testing in North Carolina. N Engl J Med 2005;352:1873–83. - PubMed

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[1] Dorfman R. The detection of defective members of large populations. Ann Math Statist 1943;14:436–40.

[2] Dorfman R. The detection of defective members of large populations. Ann Math Statist 1943;14:436–40.

[3] Bilder CR, Tebbs JM. Pooled-testing procedures for screening high volume clinical specimens in heterogeneous populations. Statist Med 2012;31:3261–8. - PMC - PubMed

[4] Bilder CR, Tebbs JM. Pooled-testing procedures for screening high volume clinical specimens in heterogeneous populations. Statist Med 2012;31:3261–8. - PMC - PubMed

[5] Erman M. What is pooled testing for COVID-19 and how can it help fight the virus? https://www.reuters.com/article/us-health-coronavirus-testing-explainer-... (Accessed August 2020).

[6] Erman M. What is pooled testing for COVID-19 and how can it help fight the virus? https://www.reuters.com/article/us-health-coronavirus-testing-explainer-... (Accessed August 2020).

[7] Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, et al. A new coronavirus associated with human respiratory disease in China. Nature 2020;579:265–9. - PMC - PubMed

[8] Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, et al. A new coronavirus associated with human respiratory disease in China. Nature 2020;579:265–9. - PMC - PubMed

[9] Pilcher CD, Fiscus SA, Nguyen TQ, Foust E, Wolf L, Williams D, et al. Detection of acute infections during HIV testing in North Carolina. N Engl J Med 2005;352:1873–83. - PubMed

[10] Pilcher CD, Fiscus SA, Nguyen TQ, Foust E, Wolf L, Williams D, et al. Detection of acute infections during HIV testing in North Carolina. N Engl J Med 2005;352:1873–83. - PubMed

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Concentrating Pooled COVID-19 Patient Lysates to Improve Reverse Transcription Quantitative PCR Sensitivity and Efficiency

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Concentrating Pooled COVID-19 Patient Lysates to Improve Reverse Transcription Quantitative PCR Sensitivity and Efficiency

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