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. 2021 Mar 31:29:e20200770.
doi: 10.1590/1678-7757-2020-0770. eCollection 2021.

Fcγ receptors on aging neutrophils

Thaís Helena Gasparoto  1 , Thalita Marcato Dalboni  1 , Nádia Ghinelli Amôr  1 , Aneli Eiko Abe  1 , Graziela Perri  1 , Vanessa Soares Lara  2 , Narciso Almeida Vieira  3 , Carlos Teodoro Gasparoto  4 , Ana Paula Campanelli  1
Affiliations

Fcγ receptors on aging neutrophils

Thaís Helena Gasparoto et al. J Appl Oral Sci. .

Abstract

Objective: Neutrophils are key effector cells of the innate immune system. They recognize antigens through membrane receptors, which are expressed during their maturation and activation. Neutrophils express FcγRII (CD32), FcγRIII (CD16), and FcγRI (CD64) after being activated by different factors such as cytokines and bacterial products. These receptors are involved with phagocytosis of IgG-opsonized microbes and enhance defense mechanisms. Based on that, our study seeks to compare the expression of FcγRII, FcγRIII, FcγRI, and CD11b on neutrophils from elderly and young subjects and their expression after in vitro activation with cytokines and LPS.

Methodology: Neutrophils were isolated from human peripheral blood and from mice bone marrow by density gradient. After isolation, FCγRs expression was immediately analyzed by flow cytometry or after in vitro stimulation.

Results: In freshly isolated cells, the percentage of FcγRIIIb+ and CD11b+ neutrophils were higher in samples from young individuals; FcγRIIIa expression was more prominent on aged neutrophils; FcγRIA expression was similar in all samples analyzed. Exposure to CXCL8 and LPS resulted in a higher percentage of FcγRIa+ neutrophils on elderly individuals' samples but lower when compared with neutrophils from young donors. We observed that LPS caused an increase in FcγRIIa expression on aging human neutrophils. In contrast, FcγRIIIb expression in response to CXCL8 and LPS stimulation was not altered in the four groups. CD11b expression was lower in neutrophils from elderly individuals even in response to LPS and CXCL8. In mice, we observed differences only regarding CD11b expression, which was increased on aged neutrophils. LPS exposure caused an increase in all FcγRs.

Conclusions: Our results suggest that, in humans, the overall pattern of FcγR expression and integrin CD11b are altered during aging and immunosenescence might contribute to age-related infection.

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Conflict of interest statement

Conflict of interest

The authors report no conflicts of interest related to this study.

Declaration of competing interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 2
Figure 2. Young human samples present higher FcγR+ neutrophils than older adults’ samples after in vitro activation.
Figure 3
Figure 3. Aged mice have higher frequency of CD11b+ neutrophils.
Figure 4
Figure 4. Young murine samples have higher FcγRI+ and CD11+ neutrophils than aged mice after in vitro activation.
Figure 1
Figure 1. CXCL8 levels and expression of FcγRIIIb and CD11b on neutrophils from healthy elderly individuals are decreased.
See this image and copyright information in PMC

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