Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation"

Channabasavaiah B Gurumurthy^{1

2}, Aidan R O'Brien^{3

4}, Rolen M Quadros⁵, John Adams Jr⁶, Pilar Alcaide⁷, Shinya Ayabe⁸, Johnathan Ballard⁶, Surinder K Batra⁹, Marie-Claude Beauchamp¹⁰, Kathleen A Becker¹¹, Guillaume Bernas¹², David Brough¹³, Francisco Carrillo-Salinas⁷, Wesley Chan¹⁰, Hanying Chen¹⁴, Ruby Dawson¹⁵, Victoria DeMambro¹¹, Jinke D'Hont^{16

17}, Katharine Dibb¹⁸, James D Eudy¹⁹, Lin Gan²⁰, Jing Gao⁴, Amy Gonzales⁶, Anyonya Guntur¹¹, Huiping Guo⁶, Donald W Harms⁵, Anne Harrington¹¹, Kathryn E Hentges²¹, Neil Humphreys²², Shiho Imai²³, Hideshi Ishii²⁴, Mizuho Iwama⁸, Eric Jonasch²⁵, Michelle Karolak¹¹, Bernard Keavney²⁶, Nay-Chi Khin⁴, Masamitsu Konno²⁷, Yuko Kotani²⁸, Yayoi Kunihiro²⁸, Imayavaramban Lakshmanan⁹, Catherine Larochelle²⁹, Catherine B Lawrence¹³, Lin Li³⁰, Volkhard Lindner¹¹, Xian-De Liu²⁵, Gloria Lopez-Castejon³¹, Andrew Loudon³², Jenna Lowe⁴, Loydie Jerome-Majeweska¹⁰, Taiji Matsusaka²³, Hiromi Miura^{33

34}, Yoshiki Miyasaka²⁸, Benjamin Morpurgo⁶, Katherine Motyl¹¹, Yo-Ichi Nabeshima³⁵, Koji Nakade⁸, Toshiaki Nakashiba⁸, Kenichi Nakashima⁸, Yuichi Obata⁸, Sanae Ogiwara³⁶, Mariette Ouellet¹², Leif Oxburgh¹¹, Sandra Piltz¹⁵, Ilka Pinz¹¹, Moorthy P Ponnusamy⁹, David Ray³⁷, Ronald J Redder¹⁹, Clifford J Rosen¹¹, Nikki Ross⁴, Mark T Ruhe³⁸, Larisa Ryzhova¹¹, Ane M Salvador⁷, Sabrina Shameen Alam¹⁰, Radislav Sedlacek³⁹, Karan Sharma⁴⁰, Chad Smith²⁵, Katrien Staes^{16

17}, Lora Starrs⁴, Fumihiro Sugiyama⁴¹, Satoru Takahashi⁴¹, Tomohiro Tanaka⁴², Andrew Trafford¹⁸, Yoshihiro Uno²⁸, Leen Vanhoutte^{16

17}, Frederique Vanrockeghem^{16

17}, Brandon J Willis³⁸, Christian S Wright⁴³, Yuko Yamauchi²⁸, Xin Yi⁴³, Kazuto Yoshimi²⁸, Xuesong Zhang²⁵, Yu Zhang³⁰, Masato Ohtsuka^{33

34}, Satyabrata Das⁴⁴, Daniel J Garry^{44

45}, Tino Hochepied^{16

17}, Paul Thomas¹⁵, Jan Parker-Thornburg²⁵, Antony D Adamson²², Atsushi Yoshiki⁸, Jean-Francois Schmouth¹², Andrei Golovko⁶, William R Thompson⁴³, K C Kent Lloyd^{38

46}, Joshua A Wood³⁸, Mitra Cowan⁴⁷, Tomoji Mashimo²⁸, Seiya Mizuno⁴¹, Hao Zhu³⁰, Petr Kasparek³⁹, Lucy Liaw¹¹, Joseph M Miano²⁰, Gaetan Burgio⁴⁸

Affiliations

¹ Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.
² Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.
³ Transformational Bioinformatics, Health and Biosecurity Business Unit, CSIRO, Sydney, Australia.
⁴ Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia.
⁵ Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA.
⁶ Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA.
⁷ Department of Immunology, Tufts University School of Medicine, Boston, USA.
⁸ RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan.
⁹ Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
¹⁰ Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada.
¹¹ Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA.
¹² Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada.
¹³ Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, AV Hill Building, Oxford Road, Manchester, M13 9PT, UK.
¹⁴ School of Medicine, Indiana University, Indianapolis, IN, 46202, USA.
¹⁵ South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia.
¹⁶ Transgenic mouse core facility, VIB Center for Inflammation Research, Ghent, Belgium.
¹⁷ Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
¹⁸ Unit of Cardiac Physiology, School of Medical Sciences, Manchester Academic Health Science Center, University of Manchester, Manchester, UK.
¹⁹ High-Throughput DNA Sequencing and Genotyping Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA.
²⁰ University of Rochester Medical Center, Rochester, NY, 14642, USA.
²¹ Division of Evolution and Genomic Sciences, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK.
²² Transgenic Unit core facility, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
²³ Department of Basic Medicine, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
²⁴ Department of Medical Data Science, Osaka University Graduate School of Medicine, Suita, Japan.
²⁵ The University of Texas, MD Anderson Cancer Center, Houston, TX, USA.
²⁶ Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester AND Manchester Heart Centre, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
²⁷ Department of Frontier Science for Cancer and Chemotherapy, Osaka University Graduate School of Medicine, Osaka, Japan.
²⁸ The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
²⁹ Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada.
³⁰ Children's Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
³¹ Manchester Collaborative Centre for Inflammation Research (MCCIR), School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK.
³² Centre for Biological Timing, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
³³ Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
³⁴ Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
³⁵ Laboratory of Molecular Life Science, Foundation for Biomedical Research and Innovation, Kobe, Japan.
³⁶ Department of Laboratory Animal Science, Support Center for Medical Research and Education, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
³⁷ Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, OX37LE, UK.
³⁸ Mouse Biology Program, University of California, Davis, USA.
³⁹ Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
⁴⁰ College of Osteopathic Medicine, Marian University, Indianapolis, IN, 46222, USA.
⁴¹ Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan.
⁴² Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
⁴³ Department of Physical Therapy, School of Health and Human Sciences, Indiana University, Indianapolis, IN, 46202, USA.
⁴⁴ Lillehei Heart Institute Regenerative Medicine and Sciences Program, University of Minnesota, Minneapolis, MN, USA.
⁴⁵ Paul and Sheila Wellstone Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN, USA.
⁴⁶ Department of Surgery, School of Medicine, University of California, Davis, Davis, USA.
⁴⁷ McGill Integrated Core for Animal Modeling (MICAM), Montreal, Canada.
⁴⁸ Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia. gaetan.burgio@anu.edu.au.

PMID: 33827648
PMCID: PMC8025318
DOI: 10.1186/s13059-021-02320-3

Comment

Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation"

Channabasavaiah B Gurumurthy et al. Genome Biol. 2021.

. 2021 Apr 7;22(1):99.

doi: 10.1186/s13059-021-02320-3.

Authors

Affiliations

¹ Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.
² Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.
³ Transformational Bioinformatics, Health and Biosecurity Business Unit, CSIRO, Sydney, Australia.
⁴ Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia.
⁵ Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA.
⁶ Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, TX, 77843, USA.
⁷ Department of Immunology, Tufts University School of Medicine, Boston, USA.
⁸ RIKEN BioResource Research Center, Tsukuba, Ibaraki, 305-0074, Japan.
⁹ Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA.
¹⁰ Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada.
¹¹ Maine Medical Center Research Institute (MMCRI), Scarborough, ME, USA.
¹² Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada.
¹³ Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, AV Hill Building, Oxford Road, Manchester, M13 9PT, UK.
¹⁴ School of Medicine, Indiana University, Indianapolis, IN, 46202, USA.
¹⁵ South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia.
¹⁶ Transgenic mouse core facility, VIB Center for Inflammation Research, Ghent, Belgium.
¹⁷ Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
¹⁸ Unit of Cardiac Physiology, School of Medical Sciences, Manchester Academic Health Science Center, University of Manchester, Manchester, UK.
¹⁹ High-Throughput DNA Sequencing and Genotyping Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA.
²⁰ University of Rochester Medical Center, Rochester, NY, 14642, USA.
²¹ Division of Evolution and Genomic Sciences, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK.
²² Transgenic Unit core facility, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
²³ Department of Basic Medicine, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
²⁴ Department of Medical Data Science, Osaka University Graduate School of Medicine, Suita, Japan.
²⁵ The University of Texas, MD Anderson Cancer Center, Houston, TX, USA.
²⁶ Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester AND Manchester Heart Centre, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
²⁷ Department of Frontier Science for Cancer and Chemotherapy, Osaka University Graduate School of Medicine, Osaka, Japan.
²⁸ The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
²⁹ Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada.
³⁰ Children's Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
³¹ Manchester Collaborative Centre for Inflammation Research (MCCIR), School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK.
³² Centre for Biological Timing, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
³³ Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
³⁴ Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
³⁵ Laboratory of Molecular Life Science, Foundation for Biomedical Research and Innovation, Kobe, Japan.
³⁶ Department of Laboratory Animal Science, Support Center for Medical Research and Education, Tokai University, 143, Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
³⁷ Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, OX37LE, UK.
³⁸ Mouse Biology Program, University of California, Davis, USA.
³⁹ Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
⁴⁰ College of Osteopathic Medicine, Marian University, Indianapolis, IN, 46222, USA.
⁴¹ Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan.
⁴² Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
⁴³ Department of Physical Therapy, School of Health and Human Sciences, Indiana University, Indianapolis, IN, 46202, USA.
⁴⁴ Lillehei Heart Institute Regenerative Medicine and Sciences Program, University of Minnesota, Minneapolis, MN, USA.
⁴⁵ Paul and Sheila Wellstone Muscular Dystrophy Center, University of Minnesota, Minneapolis, MN, USA.
⁴⁶ Department of Surgery, School of Medicine, University of California, Davis, Davis, USA.
⁴⁷ McGill Integrated Core for Animal Modeling (MICAM), Montreal, Canada.
⁴⁸ Department of Immunology and Infectious Disease, the John Curtin School of Medical Research, The Australian National University, Canberra, Australia. gaetan.burgio@anu.edu.au.

PMID: 33827648
PMCID: PMC8025318
DOI: 10.1186/s13059-021-02320-3

No abstract available

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Comment on

One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering.
Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R. Yang H, et al. Cell. 2013 Sep 12;154(6):1370-9. doi: 10.1016/j.cell.2013.08.022. Epub 2013 Aug 29. Cell. 2013. PMID: 23992847 Free PMC article.
Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation.
Gurumurthy CB, O'Brien AR, Quadros RM, Adams J Jr, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J, Dibb KM, Eudy JD, Gan L, Gao J, Gonzales A, Guntur AR, Guo H, Harms DW, Harrington A, Hentges KE, Humphreys N, Imai S, Ishii H, Iwama M, Jonasch E, Karolak M, Keavney B, Khin NC, Konno M, Kotani Y, Kunihiro Y, Lakshmanan I, Larochelle C, Lawrence CB, Li L, Lindner V, Liu XD, Lopez-Castejon G, Loudon A, Lowe J, Jerome-Majewska LA, Matsusaka T, Miura H, Miyasaka Y, Morpurgo B, Motyl K, Nabeshima YI, Nakade K, Nakashiba T, Nakashima K, Obata Y, Ogiwara S, Ouellet M, Oxburgh L, Piltz S, Pinz I, Ponnusamy MP, Ray D, Redder RJ, Rosen CJ, Ross N, Ruhe MT, Ryzhova L, Salvador AM, Alam SS, Sedlacek R, Sharma K, Smith C, Staes K, Starrs L, Sugiyama F, Takahashi S, Tanaka T, Trafford AW, Uno Y, Vanhoutte L, Vanrockeghem F, Willis BJ, Wright CS, Yamauchi Y, Yi X, Yoshimi K, Zhang X, Zhang Y, Ohtsuka M, Das S, Garry DJ, Hochepied T, Thomas P, Parker-Thornburg J, Adamson AD, Yoshiki A, Schmouth JF, Golovko A, Thompson WR, Lloyd KCK, Wood JA, Cowan M, Mashimo T, Mizuno S, Zhu H, Kasparek P, Lia… See abstract for full author list ➔ Gurumurthy CB, et al. Genome Biol. 2019 Aug 26;20(1):171. doi: 10.1186/s13059-019-1776-2. Genome Biol. 2019. PMID: 31446895 Free PMC article.
Response to "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation".
Yang H, Wang H, Jaenisch R. Yang H, et al. Genome Biol. 2021 Apr 7;22(1):98. doi: 10.1186/s13059-021-02312-3. Genome Biol. 2021. PMID: 33827646 Free PMC article. No abstract available.

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Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation"

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Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation"

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