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. 2021 Oct;7(5):763-771.
doi: 10.1002/cre2.420. Epub 2021 Apr 7.

Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self-etching primers/adhesives

Stefanie Amend  1 , Roland Frankenberger  2 , Christina Boutsiouki  1 , Vanessa Scharrelmann  1 , Julia Winter  2 , Norbert Krämer  1
Affiliations

Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self-etching primers/adhesives

Stefanie Amend et al. Clin Exp Dent Res. 2021 Oct.

Abstract

Background: It is still not fully understood what pretreatment is best for achieving maximum tightness for pit and fissure sealings (PFS).

Aim: This study investigated microleakage of PFS placed after etching with phosphoric acid or after the application of self-etching primers/adhesives (SEPA).

Design: 131 third molars were assigned to ten groups. In Hel-P, Helioseal® was applied after phosphoric acid etching. In the other groups, SEPA were used (Dyr-AP: Adper™ Prompt™ L-Pop™, Dyract® Seal; Bea: BeautiSealant Primer and Paste; Hel-Exp: Experimental primer, Helioseal®; Hel-Cl: Clearfil™ SE Bond1 , Helioseal®). Specimens were stored in distilled water at 37°C (28 days), followed by 3500 thermocycles and staining with 5% methylene blue (M) or 5% silver nitrate (S). After methylene blue staining and sectioning, microleakage was assessed light microscopically. During silver nitrate staining, specimens were dissolved by 32% HCl and remaining PFS were evaluated under a SEM.

Results: Tightness, percentage of penetrated area, and maximum dye penetration were best for Hel-P and Hel-Cl (p < 0.05).

Conclusions: Phosphoric acid etching of enamel and Clearfil™ SE Bond resulted in the best sealing quality. Methylene blue staining allowed the evaluation of more criteria (fissure shape, voids, sealant penetration depth) compared to silver nitrate.

Keywords: etch-and-rinse; microleakage; pit and fissure sealing; self-etch.

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Conflict of interest statement

SA, CB, VS, and JW have no conflict of interest. RF and NK got research grants and speaker honorarium from Ivoclar and Dentsply.

Figures

FIGURE 1
FIGURE 1
Evaluation criteria for the methylene blue penetration test
FIGURE 2
FIGURE 2
Exemplary illustration of the percentage of penetration area of the pit and fissure sealant. On image 2a, the percentage of penetrated area is small, whereas, it is high on image 2b
FIGURE 3
FIGURE 3
To compare the results of both dye penetration tests, the maximum dye penetration depth was assessed for specimens after the methylene blue penetration test (a), and after the silver nitrate penetration test (b)
See this image and copyright information in PMC

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