Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2021 Jul;118(7):2393-2400.
doi: 10.1002/bit.27782. Epub 2021 Apr 23.

Optically transparent electrodes to study living cells: A mini review

Artem N Kuzovlev  1 , Anatoly K Evseev  2 , Irina V Goroncharovskaya  2 , Aslan K Shabanov  1   2 , Sergey S Petrikov  2
Affiliations
Review

Optically transparent electrodes to study living cells: A mini review

Artem N Kuzovlev et al. Biotechnol Bioeng. 2021 Jul.

Abstract

The use of electrochemical methods to study living systems, including cells, has been of interest to researchers for a long time. Thus, controlling the polarization of the electrode contacting living cells, one can influence, for example, their proliferation or the synthesis of specific proteins. Moreover, the electrochemical approach formed the basis of the biocompatibility improvement of the materials contacting with body tissues that use in carbon hemosorbents and implants development. It became possible to reach a fundamentally new level in the study of cell activity with the introduction of optically transparent electrodes in this area. The advantage of the using of optically transparent electrodes is the possibility of simultaneous analysis of living cells by electrochemical and microscopic methods. The use of such materials allowed approaching to the study of the influence of the electrode potential on adhesion activity and morphology of the different cell types (HeLa cells, endothelial cell, etc.) more detailed. There are a negligible number of publications in this area despite the advantages of the usage of optically transparent electrodes to study living cells. This mini-review is devoted to some aspects of the interaction of living cells with conductive materials and current advances in the use of optically transparent electrodes for the study of living cells, as well as the prospects for their use in cellular technologies.

Keywords: cell adhesion; indium tin oxide; living cell; morphology; optically transparent electrode; polarization.

PubMed Disclaimer

References

REFERENCES

    1. Afre, R. A., Sharma, N., Sharon, M., & Sharo, M. (2018). Transparent conducting oxide films for various applications: A review. Reviews on Advanced Materials Science, 53(1), 79-89. https://doi.org/10.1515/rams-2018-0006
    1. Bi, Y. G., Liu, Y. F., Zhang, X. L., Yin, D., Wang, W. Q., Feng, J., & Sun, H. B. (2019). Ultrathin metal films as the transparent electrode in ITO-free organic optoelectronic devices. Advanced Optical Materials, 7(6), 1800778. https://doi.org/10.1002/adom.201800778
    1. Bond, D. R., & Lovley, D. R. (2003). Electricity production by Geobacter sulfurreducens attached to electrodes. Applied and Environmental Microbiology, 69(3), 1548-1555. https://doi.org/10.1128/AEM.69.3.1548-1555.2003
    1. Busalmen, J. P., & de Sánchez, S. R. (2005). Electrochemical polarization-induced changes in the growth of individual cells and biofilms of Pseudomonas fluorescens (ATCC 17552). Applied and Environmental Microbiology, 71(10), 6235-6240. https://doi.org/10.1128/AEM.71.10.6235-6240.2005
    1. Cao, W., Li, J., Chen, H., & Xue, J. (2014). Transparent electrodes for organic optoelectronic devices: A review. Journal of Photonics for Energy, 4(1), 040990. https://doi.org/10.1117/1.JPE.4.040990

LinkOut - more resources