A resource of targeted mutant mouse lines for 5,061 genes

Abstract

The International Mouse Phenotyping Consortium reports the generation of new mouse mutant strains for over 5,000 genes, including 2,850 novel null, 2,987 novel conditional- ready, and 4,433 novel reporter alleles.

Figure 1:

Generation and impact of targeted alleles for 5,061 unique mouse genes. (a) Cumulative production progress, including all attempts (microinjection or aggregation (black), unique ES cell clones injected (red), unique genes attempted (yellow), and unique genes that achieved germline transmission (GLT; blue). For GLT, the date reflects the date of microinjection, and only reports the first instance of transmission for the small number of duplicate mutations produced. (b) Venn representation of unique gene null alleles produced by the IMPC (orange) and by the rest of the scientific community as reported in MGI (“Non- IMPC”; blue). (c) Unique gene conditional-ready alleles produced by the IMPC (orange) and by the rest of the scientific community (blue). (d) Unique gene reporter alleles produced by the IMPC (orange) and by the rest of the scientific community (blue). (e) Cumulative mouse orders of IMPC lines processed by production centres and mouse model Repositories from 2012–2018 (blue line). The cumulative number of ordering investigators, unique alleles ordered, and unique genes ordered are shown in yellow, grey, and orange, respectively.