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. 2021 Apr;26(14):2000131.
doi: 10.2807/1560-7917.ES.2021.26.14.2000131.

A cross-border outbreak of Salmonella Bareilly cases confirmed by whole genome sequencing, Czech Republic and Slovakia, 2017 to 2018

Klára Labská  1   2 , Michaela Špačková  1 , Ondřej Daniel  1 , Josef Včelák  3 , Veronika Vlasáková  4 , Tomáš Černý  5 , Tereza Gelbíčová  6 , Martina Florianová  6 , Renáta Karpíšková  6 , Dagmar Gavačová  7 , Mária Štefkovičová  8 , Hana Orlíková  1
Affiliations

A cross-border outbreak of Salmonella Bareilly cases confirmed by whole genome sequencing, Czech Republic and Slovakia, 2017 to 2018

Klára Labská et al. Euro Surveill. 2021 Apr.

Abstract

In August 2017, an increased incidence of Salmonella Bareilly was detected in the Czech Republic. An investigation was conducted with Slovakia to confirm the outbreak and identify the source. Probable outbreak cases were defined as cases with laboratory-confirmed S. Bareilly reported in either of the national surveillance systems, and/or the Czech and Slovak National Reference Laboratory databases from July 2017. Confirmed cases had the pulsed-field gel electrophoresis (PFGE) outbreak pulsotype or up to 5 alleles difference from outbreak cluster members by core genome multilocus sequence typing (cgMLST). PFGE and whole genome sequencing were used for isolate comparison. The same trawling questionnaire was used in both countries. By the end of October 2018, 325 cases were identified. Among 88 human S. Bareilly isolates analysed by PFGE, 82 (93%) shared an identical pulsotype; cgMLST of 17 S. Bareilly human isolates showed 1-2 allele difference. The trawling questionnaire excluded consumption of unusual or imported foods. In September 2018, an isolate closely related to the outbreak isolates was identified in a powdered egg product. A spray dryer was recognised as the contamination source and the production plant was closed. Using molecular typing methods, we detected a diffuse cross-border outbreak caused by S. Bareilly.

Keywords: Salmonella Bareilly; WGS; food- and water-borne diseases; outbreak; powdered egg product; spray dryer.

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Conflict of interest statement

Conflict of interest: None declared.

Figures

Figure 1
Figure 1
Distribution of probable and confirmed cases of Salmonella Bareilly according to case definition, by week of symptom onset in (A) Czech Republic and (B) Slovakia, 1 January 2017–31 December 2018 (n = 325)
Figure 2
Figure 2
Map displaying the numbers of Salmonella Bareilly cases and the notification rates by region, Czech Republic and Slovakia, 1 July 2017–31 October 2018 (n = 325)
Figure 3
Figure 3
Age and sex distribution of Salmonella Bareilly probable and confirmed outbreak cases, Czech Republic and Slovakia, 1 July 2017–31 October 2018 (n =325)
Figure 4
Figure 4
The dendrogram of detected Salmonella Bareilly PFGE pulsotypes, Czech Republic and Slovakia, 2016–2018
Figure 5
Figure 5
The cgMLST minimum spanning tree based on Czech outbreak isolates (indicated in yellow) and strains belonging to cgMLST type HC200-21660 (56 strains in total) from the EnteroBase, 22 January 2019
Figure 6
Figure 6
SNP- based phylogeny of Salmonella Bareilly strains (the same dataset as used for cgMLST analysis) using the SNP-Project in EnteroBase, 21 January 2019
See this image and copyright information in PMC

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