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. 2021 Apr 7;44(2):e20200083.
doi: 10.1590/1678-4685-GMB-2020-0083. eCollection 2021.

Cytogenetic and molecular characteristics of Potamotrygon motoro and Potamotrygon sp. (Chondrichthyes, Myliobatiformes, Potamotrygonidae) from the Amazon basin: Implications for the taxonomy of the genus

Vanessa Paes da Cruz  1 , Maria Ligia Oliveira Nobile  1 , Fabilene Gomes Paim  1 , Aisni Mayumi Correia de Lima Adachi  1 , Giovana da Silva Ribeiro  1 , Daniela Carvalho Ferreira  2 , José Carlos Pansonato-Alves  1 , Patrícia Charvet  3   4 , Claudio Oliveira  1 , Fausto Foresti  1
Affiliations

Cytogenetic and molecular characteristics of Potamotrygon motoro and Potamotrygon sp. (Chondrichthyes, Myliobatiformes, Potamotrygonidae) from the Amazon basin: Implications for the taxonomy of the genus

Vanessa Paes da Cruz et al. Genet Mol Biol. .

Erratum in

  • Erratum.
    [No authors listed] [No authors listed] Genet Mol Biol. 2021 May 3;44(2):e20200083er. doi: 10.1590/1678-4685-GMB-2020-0083er. Genet Mol Biol. 2021. PMID: 33957664 Free PMC article.

Abstract

The chromosomes of two freshwater stingrays, Potamotrygon motoro and Potamotrygon sp., from the Amazon River basin in Brazil were investigated using integrated molecular (cytochrome c oxidase subunit 1) and cytogenetic analyses. Potamotrygon motoro presented intraspecific variation in the diploid number, with 2n=66 in the females and 2n=65 in the males, while Potamotrygon sp. had a karyotype with 66 chromosomes, in both sexes. The C-banding revealed the presence of heterochromatic blocks accumulated in the centromeric region of all the chromosomes in both species. The FISH assays with 18S DNA probes highlighted the terminal region of three or four chromosome pairs in P. motoro and seven chromosomes in Potamotrygon sp. The rDNA 5S sequences were found in only one chromosomal pair in both species. The interspecific genetic distance based on the COI sequences, between P. motoro and Potamotrygon sp. from Amazon River was 10.8%, while that between the Amazonian P. motoro and Potamotrygon amandae from the Paraná River was 2.2%, and the genetic distance between Potamotrygon sp. and P. amandae was 11.8%. In addition to the new insights on the cytogenetics of the study species, the results of the present study confirmed the existence of heteromorphic sex-linked chromosomes in P. motoro.

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Conflict of interest statement

Conflict of Interest: The authors declare that they have no conflicts of interest. The funding agencies played no role in the design of the study, the collection, analysis or interpretation of the data, the preparation of the manuscript or the decision to publish the results.

Figures

Figure 1 -
Figure 1 -. Karyotypes of (a) male and (b) female Potamotrygon motoro after C-banding. Karyotype of Potamotrygon sp. stained with (c) Giemsa and (d) after C-banding. Scale bar =10 µm.
Figure 2 -
Figure 2 -. (a) Metaphase of P. motoro showing the 5S rDNA (red) and 18S (green) fluorescent signals; (b) Metaphase of Potamotrygon sp. showing the rDNA 5S (green) and 18S (red) fluorescent signals.
Figure 3 -
Figure 3 -. Neighbor-Joining tree of the COI gene sequence and the sex chromosome systems found in potamotrygonins species, including Potamotrygon amandae from the Paraná basin (purple), P. motoro (blue) and Potamotrygon sp. from the Amazon basin, and P. histrix (JN184071 “P. hystrix”) (green). The bootstrap values are shown at the branch nodes.
See this image and copyright information in PMC

References

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