Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar 25:12:611274.
doi: 10.3389/fmicb.2021.611274. eCollection 2021.

Dissemination of bla NDM- 1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids

José Eduardo Toledano-Tableros  1   2 , Catalina Gayosso-Vázquez  1 , Ma Dolores Jarillo-Quijada  1 , José Luis Fernández-Vázquez  1 , Rayo Morfin-Otero  3 , Eduardo Rodríguez-Noriega  3 , Silvia Giono-Cerezo  2 , Gabriel Gutkind  4 , José Di Conza  4 , José Ignacio Santos-Preciado  1 , María Dolores Alcántar-Curiel  1
Affiliations

Dissemination of bla NDM- 1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids

José Eduardo Toledano-Tableros et al. Front Microbiol. .

Abstract

Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1-producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56-60% (45-48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were bla NDM- 1 carriers and negative for bla KPC, bla NDM, bla IMP, bla VIM and bla OXA- 48 -like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82-195 kbp were carriers of bla NDM- 1, bla CTX-M- 15, bla TEM- 1, aac(6')-Ib and/or aac(3')-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying bla NDM- 1 in Mexico. This study demonstrates that bla NDM- 1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of bla NDM- 1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital.

Keywords: IncF-like plasmids; Klebsiella pneumoniae; MLST; Mexico; blaNDM–1; carbapenems.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Restriction fragment length polymorphism analysis of conjugative plasmids carrying antimicrobial resistance genes. N: blaNDM–1, C: blaCTX–M–15, T: blaTEM–1, A6: aac(6′)-Ib, A3: aac(3′)-IIa.
FIGURE 2
FIGURE 2
Phylogenetic analysis of STs obtained for blaNDM–1-producing K. pneumoniae strains using the neighbor-joining method.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Alcántar-Curiel M. D., Fernández-Vázquez J. L., Toledano-Tableros J. E., Gayosso-Vázquez C., Jarillo-Quijada M. D., López-Álvarez M. D. R., et al. (2019a). Emergence of IncFIA plasmid-carrying blaNDM–1 among Klebsiella pneumoniae and Enterobacter cloacae isolates in a tertiary referral hospital in Mexico. Microb. Drug Resist. 25 830–838. - PubMed
    1. Alcántar-Curiel M. D., Rosales-Reyes R., Jarillo-Quijada M. D., Gayosso-Vázquez C., Fernández-Vázquez J. L., Toledano-Tableros J. E., et al. (2019b). Carbapenem-resistant Acinetobacter baumannii in three tertiary care hospitals in Mexico: virulence profiles, innate immune response and clonal dissemination. Front. Microbiol. 10:2116. 10.3389/fmicb.2019.02116 - DOI - PMC - PubMed
    1. Aquino-Andrade A., Merida-Vieyra J., De La Garza E. A., Arzate-Barbosa P., Ranero A. D. C. (2018). Carbapenemase-producing Enterobacteriaceae in Mexico: report of seven non-clonal cases in a pediatric hospital. BMC Microbiol. 18:38. 10.1186/s12866-018-1166-z - DOI - PMC - PubMed
    1. Barrios H., Silva-Sanchez J., Reyna-Flores F., Sanchez-Perez A., Sanchez-Francia D., Aguirre-Torres J. A., et al. (2014). Detection of a NDM-1-producing Klebsiella pneumoniae (ST22) clinical isolate at a pediatric hospital in Mexico. Pediatr. Infect. Dis. J. 33:335. 10.1097/inf.0000000000000173 - DOI - PubMed
    1. Bocanegra-Ibarias P., Garza-Gonzalez E., Morfin-Otero R., Barrios H., Villarreal-Trevino L., Rodriguez-Noriega E., et al. (2017). Molecular and microbiological report of a hospital outbreak of NDM-1-carrying Enterobacteriaceae in Mexico. PLoS One 12:e0179651. 10.1371/journal.pone.0179651 - DOI - PMC - PubMed

LinkOut - more resources