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. 2021 Feb 6;9(4):1961-1971.
doi: 10.1002/fsn3.2162. eCollection 2021 Apr.

Functional beverages improve insulin resistance and hepatic steatosis modulating lysophospholipids in diet-induced obese rats

Julio C Rubio-Rodríguez  1 , Rosalia Reynoso-Camacho  2 , Nuria Rocha-Guzmán  3 , Luis M Salgado  1
Affiliations

Functional beverages improve insulin resistance and hepatic steatosis modulating lysophospholipids in diet-induced obese rats

Julio C Rubio-Rodríguez et al. Food Sci Nutr. .

Abstract

Hypercaloric beverages increase the prevalence of insulin resistance and nonalcoholic fatty liver disease (NAFLD), diets with polyphenolic compounds improved these alterations. The study aimed to evaluate the effect of the consumption of three functional beverages (prepared with: Roselle, green tea, cinnamon, Malabar tamarind, and peppermint in different proportions) on insulin resistance and NAFLD and their relation to liver phospholipid regulation in Wistar rats fed with a high-fat and fructose (HFF) diet. The consumption of beverages showed lower liver triglycerides compared to HFF control group, being the called beverage B the successful triggering up to 30.1%. The consumption of functional beverages improved insulin resistance and decreased the abundance of LysoPC (20:2), LysoPC (16:0), LysoPC (14:0), LysoPE (18:0), LysoPC (15:0), and LysoPC (20:1), with beverage C being the one with the meaningful effect. The results indicate that the functional beverage consumption improves insulin resistance, and decrease the degree of NAFLD, these through modifications of lysophosphatidylcholines, and lipids metabolism.

Keywords: NAFLD (nonalcoholic fatty liver disease); functional beverage; insulin resistance; lipids metabolism; lysophospholipids.

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Conflict of interest statement

Rubio‐Rodríguez Julio C, Reynoso‐Camacho Rosalia, Rocha Guzmán Nuria, and Salgado Luis M. declare that they do not have any conflict of interest. Experiments on animals were performed in accordance with the Animal Care and Use Protocol of the Autonomous University of Queretaro, as recommended by the guidelines for animal testing (NOM‐062‐ZOO‐1999).

Figures

FIGURE 1
FIGURE 1
Effect of functional beverages on liver lipids. (a) quantification of liver triglycerides Data are expressed as mean ± standard deviation (n = 8). *Indicate statistical difference (p < .05) as compared to the SD control by Dunnet test. Ꞩ Indicate statistical difference (p < .05) between HFF control and groups HFF treated with beverages by Dunnet test. (b) Liver tissue microphotography stained with hematoxylin and eosin. SD, group fed with standard diet and HFF, group fed with high fat and fructose
FIGURE 2
FIGURE 2
Multivariate analysis of liver lysophospholipids of rats fed with HFF and treated with functional beverages. Heat map (a), PCA plots analysis (b), and PLSDA plots analysis (c). Data analysis (n = 5). SD, group fed standard diet and HFF, group fed with high fat and fructose
FIGURE 3
FIGURE 3
Lysophospholipids identified based on VIP scores in liver of rats fed with HFF and functional beverages. Partial least squares discriminant analysis [PLSDA] variable importance in projection [VIP] plot in liver (a), and Intensities of lysophospholipids (VIP > 1.2) in liver (b). Data analysis (n = 5). SD, group fed with standard diet and HFF, group fed with high fat and fructose. *Indicate statistical difference (p < .05) as compared to the SD control by Dunnet test
See this image and copyright information in PMC

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