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Review
. 2021:2281:23-47.
doi: 10.1007/978-1-0716-1290-3_2.

Single-Stranded DNA-Binding Proteins in the Archaea

Najwa Taib  1   2 , Simonetta Gribaldo  1 , Stuart A MacNeill  3
Affiliations
Free article
Review

Single-Stranded DNA-Binding Proteins in the Archaea

Najwa Taib et al. Methods Mol Biol. 2021.
Free article

Abstract

Single-stranded (ss) DNA-binding proteins are found in all three domains of life where they play vital roles in nearly all aspects of DNA metabolism by binding to and stabilizing exposed ssDNA and acting as platforms onto which DNA-processing activities can assemble. The ssDNA-binding factors SSB and RPA are extremely well conserved across bacteria and eukaryotes, respectively, and comprise one or more OB-fold ssDNA-binding domains. In the third domain of life, the archaea, multiple types of ssDNA-binding protein are found with a variety of domain architectures and subunit compositions, with OB-fold ssDNA-binding domains being a characteristic of most, but not all. This chapter summarizes current knowledge of the distribution, structure, and biological function of the archaeal ssDNA-binding factors, highlighting key features shared between clades and those that distinguish the proteins of different clades from one another. The likely cellular functions of the proteins are discussed and gaps in current knowledge identified.

Keywords: Archaea; DNA repair; DNA replication; OB fold; Phylogenetics; RPA; Replication protein A; SSB; Single-stranded DNA; ssDNA.

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References

    1. Marceau AH (2012) Functions of single-strand DNA-binding proteins in DNA replication, recombination, and repair. Methods Mol Biol 922:1–21 - PubMed
    1. Theobald DL, Mitton-Fry RM, Wuttke DS (2003) Nucleic acid recognition by OB-fold proteins. Annu Rev Biophys Biomol Struct 32:115–133 - PubMed - PMC - DOI
    1. Bianco PR (2017) The tale of SSB. Prog Biophys Mol Biol 127:111–118 - PubMed - DOI - PMC
    1. Eggington JM, Haruta N, Wood EA, Cox MM (2004) The single-stranded DNA-binding protein of Deinococcus radiodurans. BMC Microbiol 4:2 - PubMed - PMC - DOI
    1. Bernstein DA, Eggington JM, Killoran MP, Misic AM, Cox MM, Keck JL (2004) Crystal structure of the Deinococcus radiodurans single-stranded DNA-binding protein suggests a mechanism for coping with DNA damage. Proc Natl Acad Sci U S A 101:8575–8580 - PubMed - PMC - DOI