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. 1988 Jul;170(7):3287-90.
doi: 10.1128/jb.170.7.3287-3290.1988.

Mechanism of biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme

T Nesbakken  1 , P Kolsaker, J Ormerod
Affiliations

Mechanism of biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme

T Nesbakken et al. J Bacteriol. 1988 Jul.

Abstract

The biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme was investigated by 13C nuclear magnetic resonance spectroscopy of the oxoacid formed from 13C-labeled acetate by washed suspensions. The threonine pathway could be excluded, and the results are in accord with a mechanism for the formation of 2-oxobutyrate from acetyl coenzyme A and pyruvate via citramalate.

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