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. 2021 Apr 8:2021:10.17912/micropub.biology.000383.
doi: 10.17912/micropub.biology.000383.

Genetic Mapping of a new Hippo allele, HpoN.1.2, in Drosophila melanogaster

Jamie L Siders  1 , Kayla L Bieser  2 , Danielle R Hamill  3 , Erika C Acosta  2 , Olivia K Alexander  1 , Humza I Ali  4 , Micah J Anderson  1 , Hayden R Arrasmith  1 , Mustafa Azam  4 , Nikki J Beeman  2 , Hassan Beydoun  4 , Lauren J Bishop  1 , Morgan D Blair  1 , Brianna Bletch  4 , Heather R Bline  1 , Jennifer C Brown  1 , Kelly M Burns  1 , Karina C Calagua  2 , Lexie Chafin  3 , William Ah Christy  3 , Carlyn Ciamacco  1 , Hannah Cizauskas  4 , Caitlyn M Colwell  3 , Abigail R Courtright  3 , Lucero Diaz Alavez  2 , Rayne Is Ecret  2 , Fatima Edriss  4 , Taylor G Ellerbrock  1 , Madison M Ellis  1 , Erica M Extine  1 , Eric Feldman  2 , Luke J Fickenworth  1 , Caroline M Goeller  1 , Alexis S Grogg  1 , Yailine Hernandez  2 , Abigail Hershner  1 , Megan M Jauss  1 , Leyre Jimenez Garcia  1 , Katey E Franks  1 , Ethan T Kazubski  1 , Emily R Landis  1 , Jon Langub  2 , Tia N Lassek  2 , Triet C Le  1 , Julia M Lee  1 , Daniel P Levine  2 , Phoebe J Lightfoot  3 , Natasha Love  2 , Ali Maalhagh-Fard  4 , Colin Maguire  1 , Brynna E McGinnis  2 , Bhargavi V Mehta  1 , Veronica Melendrez  2 , Zimri E Mena  2 , Seth Mendell  4 , Petra Montiel-Garcia  2 , Autumn S Murry  4   5 , Riley A Newland  1 , Ryan M Nobles  2 , Neha Patel  2 , Yashodhara Patil  4 , Cassidy L Pfister  1 , Victoria Ramage  1 , Mya R Ray  1 , Joseph Rodrigues  2 , Victoria C Rodriquez  2 , Yara Romero  2 , Alexandra M Scott  1 , Nicholas Shaba  4 , Samantha Sieg  4 , Kayla Silva  1 , Sahiba Singh  4 , Aleksandria J Spargo  1 , Savanna J Spitnale  1 , Nicole Sweeden  2 , Logan Tague  4 , Breanna M Tavernini  2 , Kathleen Tran  1 , Liselle Tungol  4 , Kylie A Vestal  1 , Amber Wetherbee  1 , Kayla M Wright  1 , Anthony T Yeager  1 , Rehab Zahid  4 , Jacob D Kagey  4
Affiliations

Genetic Mapping of a new Hippo allele, HpoN.1.2, in Drosophila melanogaster

Jamie L Siders et al. MicroPubl Biol. .

Abstract

Genetic screens provide a mechanism to identify genes involved with different cellular and organismal processes. Using a Flp/FRT screen in the Drosophila eye we identified mutations that result in alterations and de-regulation of cell growth and division. From this screen a group of undergraduate researchers part of the Fly-CURE consortium mapped and characterized a new allele of the gene Hippo, HpoN.1.2.

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Figures

Figure 1. The<i> Dark<sup>82</sup>, Hpo<sup>N.1.2</sup></i> mosaic eye results in dramatic tissue overgrowth and pupal lethality.
Figure 1. The Dark82, HpoN.1.2 mosaic eye results in dramatic tissue overgrowth and pupal lethality.
A.) Lateral view of FRT42D, Dark82 mosaic control eye and B.) FRT42D, Dark82,HpoN.1.2 mosaic mutant pupal eyes at the same magnification (40x). In both genotypes, mutant tissue displays red pigmentation (w+mC). Arrows on A and B point to antennae, eyes are oriented with anterior to the left and dorsal to the top. C.) Genomic region of chromosome 2R in which mutant N.1.2 failed to complement (2R:19,462,450..19,506,861). The N.1.2 mutation mapped to the hpo gene locus found in this region (yellow highlight). Image adapted from flybase.org (Gramates 2017).
See this image and copyright information in PMC

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