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Review
. 2021 Mar 29:12:649878.
doi: 10.3389/fmicb.2021.649878. eCollection 2021.

Potential Role and Utilization of Plant Growth Promoting Microbes in Plant Tissue Culture

Abdoulaye Soumare  1   2   3 , Abdala G Diédhiou  2   3   4 , Naveen Kumar Arora  5 , Laith Khalil Tawfeeq Al-Ani  6   7 , Mariama Ngom  2   8 , Saliou Fall  2 , Mohamed Hafidi  1   9 , Yedir Ouhdouch  1   9 , Lamfeddal Kouisni  1 , Mame Ourèye Sy  4   8
Affiliations
Review

Potential Role and Utilization of Plant Growth Promoting Microbes in Plant Tissue Culture

Abdoulaye Soumare et al. Front Microbiol. .

Abstract

Plant growth promoting microbes (PGPMs) play major roles in diverse ecosystems, including atmospheric nitrogen fixation, water uptake, solubilization, and transport of minerals from the soil to the plant. Different PGPMs are proposed as biofertilizers, biostimulants, and/or biocontrol agents to improve plant growth and productivity and thereby to contribute to agricultural sustainability and food security. However, little information exists regarding the use of PGPMs in micropropagation such as the in vitro plant tissue culture. This review presents an overview of the importance of PGPMs and their potential application in plant micropropagation. Our analysis, based on published articles, reveals that the process of in vitro classical tissue culture techniques, under strictly aseptic conditions, deserves to be reviewed to allow vitroplants to benefit from the positive effect of PGPMs. Furthermore, exploiting the potential benefits of PGPMs will lead to lessen the cost production of vitroplants during micropropagation process and will make the technique of plant tissue culture more efficient. The last part of the review will indicate where research is needed in the future.

Keywords: agriculture; biotechnology; phytohormones; plant growth promoting bacteria; plant growth promoting fungi; plant growth promoting microbe; plant tissue culture.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Main stages of micropropagation and required chemical components for each step.
Figure 2
Figure 2
Role and mechanisms of rhizospheric plant growth promoting microbes.
Figure 3
Figure 3
Schematic representation of root and rhizosphere colonization by beneficial microorganisms. AMF, arbuscular mycorrhizal fungi; ERM, ericoid mycorrhizal fungi; OMF, orchid mycorrhizal fungi; EMF, ectomycorrhizal fungi; EF, endophyte fungi; A, arbuscules; ERH, extraradical hyphae; V, vesicles; S, spore; HC, hyphal coils; P, peloton; HN, Hartig net; and M, mantle.
Figure 4
Figure 4
Schematic summary of some benefits of biotization process compared to classical micropropagation process.
See this image and copyright information in PMC

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