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. 2021 Mar 27:2021:6666642.
doi: 10.1155/2021/6666642. eCollection 2021.

One-Pot Reducing Agent-Free Synthesis of Silver Nanoparticles/Nitrocellulose Composite Surface Coating with Antimicrobial and Antibiofilm Activities

K G U R Kumarasinghe  1 , W C H Silva  1 , M D A Fernando  1 , L Palliyaguru  1 , P S Jayawardena  2 , M Shimomura  2 , S S N Fernando  3 , T D C P Gunasekara  3 , P M Jayaweera  1
Affiliations

One-Pot Reducing Agent-Free Synthesis of Silver Nanoparticles/Nitrocellulose Composite Surface Coating with Antimicrobial and Antibiofilm Activities

K G U R Kumarasinghe et al. Biomed Res Int. .

Abstract

Nitrocellulose with silver nanoparticle (AgNP/NC) composite was prepared in situ using Ag(CH3CO2) and nitrocellulose without any reducing agent. The composite materials synthesized were spray coated onto glass substrates to obtain thin films. The AgNPs/NC composites were characterized by ultraviolet-visible, Fourier transform infrared, X-ray photoelectron spectroscopy, scanning electron microscopy, and transmission electron microscopy. The antimicrobial activity of AgNPs/NC composite was investigated by tube method and time-kill kinetic studies against three microbial species, including Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), and Candida albicans (ATCC 10231). The antibiofilm activities were qualitatively determined against all three organisms. Prepared AgNPs/NC films exhibited good antimicrobial activity and significant inhibition of biofilm development against all three microbial species. The effective dispersion of AgNPs/NC in biofilm was responsible for the significant antibiofilm activity of the prepared material. The reported AgNPs/NC composite can be used as coating additive in bacteriocidal paint which can be applied onto surfaces such as in healthcare environments.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
NC ethyl acetate mixture heated at 60°C for 1 h in the (a) absence of and (b) presence of Ag ions.
Figure 2
Figure 2
SPR peak intensity variation with reaction time at 40°C and 60°C; UV-Vis absorption spectra. NC to Ag ratio 1.0 : 0.05 (wt%).
Figure 3
Figure 3
SPR peak intensity variation with reaction time at (a) 40°C and (b) 60°C; UV-Vis absorption spectra (I) 40°C and (II) 60°C; NC to Ag ratio 1.0 : 0.05 (wt%).
Figure 4
Figure 4
UV-Vis absorption spectra (a) NC film and (b) AgNPs/NC film. Images of (i) NC and (ii) AgNPs/NC films coated onto petri dishes.
Figure 5
Figure 5
Representation of the in situ synthesis of AgNPs on NC polymer matrix.
Figure 6
Figure 6
Pseudo-first order reaction kinetic plots for the reduction of Ag ions at 40°C and 60°C.
Figure 7
Figure 7
TEM image of (a) AgNPs/NC, (b) histogram of particle size distribution, and (c) EDX analysis.
Figure 8
Figure 8
TEM image of (a) bare NC sample and (b) EDX analysis.
Figure 9
Figure 9
SEM images of film of (a) bare NC and (b) AgNPs/NC sample; EDX analysis (c) bare NC and (d) AgNPs/NC sample.
Figure 10
Figure 10
ATR-FTIR spectra of the prepared films (a) NC and (b) AgNPs/NC.
Figure 11
Figure 11
High-resolution XPS scans of NC and AgNPs/NC: (a) N 1 s, (b) O 1 s, (c) C 1 s, and (d) Ag 3d regions.
Figure 12
Figure 12
UV-Vis absorption spectra of AgNPs/NC film with time under ambient conditions.
Figure 13
Figure 13
TG and DTG curves of (a) NC and (b) AgNPs/NC film.
Figure 14
Figure 14
Inhibition of microbial growth on NC strips with and without AgNPs: NC strips labelled as without AgNPs (i), with AgNPs (ii), and in sterile nutrient broth (iii). (a–c) Strips before incubation with test organisms; (d–f) strips after incubation for 24 h with (a, d) P. aeruginosa, (b, e) S. aureus, and (c, f) C. albicans microbial species.
Figure 15
Figure 15
Time-kill assay to determine the contact time required for 100% inhibition of CFU in the test microorganisms. Bar charts represent the percentage inhibition of CFU/ml over contact time of (a) P. aeruginosa, (b) S. aureus, and (c) C. albicans microbial species.
See this image and copyright information in PMC

References

    1. Neubeiser A., Bonsignore M., Tafelski S., et al. Mortality attributable to hospital acquired infections with multidrug-resistant bacteria in a large group of German hospitals. Journal of Infection and Public Health. 2020;13(2):204–210. doi: 10.1016/j.jiph.2019.07.025. - DOI - PubMed
    1. Allegranzi B., Nejad S. B., Combescure C., et al. Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis. Lancet. 2011;377(9761):228–241. doi: 10.1016/S0140-6736(10)61458-4. - DOI - PubMed
    1. Agarwal M., Shiau S., Larson E. L. Repeat gram-negative hospital-acquired infections and antibiotic susceptibility: a systematic review. Journal of Infection and Public Health. 2018;11(4):455–462. doi: 10.1016/j.jiph.2017.09.024. - DOI - PMC - PubMed
    1. Chaoui L., Mhand R., Mellouki F., Rhallabi N. Contamination of the Surfaces of a Health Care Environment by Multidrug- Resistant (MDR) Bacteria. International Journal of Microbiology. 2019;2019:7. doi: 10.1155/2019/3236526.3236526 - DOI - PMC - PubMed
    1. Chen Y., He Y., Zhang W., et al. Pathogenic characteristics of nosocomial infections in patients with cerebrovascular diseases and characteristics and treatment of pathogenic bacteria in different seasons. Journal of Infection and Public Health. 2020;13(5):800–805. doi: 10.1016/j.jiph.2019.11.010. - DOI - PubMed

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