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. 2021 Apr 15;10(15):e00280-21.
doi: 10.1128/MRA.00280-21.

Metatranscriptomic Analysis Reveals SARS-CoV-2 Mutations in Wastewater of the Frankfurt Metropolitan Area in Southern Germany

Shelesh Agrawal  1 , Laura Orschler  2 , Susanne Lackner  2
Affiliations

Metatranscriptomic Analysis Reveals SARS-CoV-2 Mutations in Wastewater of the Frankfurt Metropolitan Area in Southern Germany

Shelesh Agrawal et al. Microbiol Resour Announc. .

Abstract

We report a sequencing analysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples collected in the Frankfurt metropolitan area of Germany. The majority of the detected mutations have been identified only in clinical genomes outside Frankfurt, indicating that the sequencing of SARS-CoV-2 RNA in wastewater can provide insights into emerging variants in a city.

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Figures

FIG 1
FIG 1
Locations of mutations detected in samples (NR, Niederrad; SL, Sindlingen; GR, Griesheim), corresponding to the locations reporting the mutations in clinical samples. The detected mutations are organized according to the corresponding gene (M, N, open reading frame 1ab [Orf1ab], Orf3a, and S) of the SARS-CoV-2 genome. Points indicate the locations reporting the detected mutations.
See this image and copyright information in PMC

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