Halomonas maris sp. nov., a moderately halophilic bacterium isolated from sediment in the southwest Indian Ocean

Abstract

A halophilic, Gram-staining-negative, rod-shaped, flagellated and motile bacterium, strain QX-1 ^T, was isolated from deep-sea sediment at a depth of 3332 m in the southwestern Indian Ocean. Strain QX-1 ^T growth was observed at 4-50 °C (optimum 37 °C), pH 5.0-11.0 (optimum pH 7.0), 3-25% NaCl (w/v; optimum 7%), and it did not grow without NaCl. A phylogenetic analysis based on the 16S rRNA gene placed strain QX-1 ^T in the genus Halomonas and most closely related to Halomonas sulfidaeris (97.9%), Halomonas zhaodongensis (97.8%), Halomonas songnenensis (97.6%), Halomonas hydrothermalis (97.4%), Halomonas subterranea (97.3%), Halomonas salicampi (97.1%), and Halomonas arcis (97.0%). DNA-DNA hybridization (< 26.5%) and average nucleotide identity values (< 83.5%) between strain QX-1 ^T and the related type strains meet the accepted criteria for a new species. The principal fatty acids (> 10%) of strain QX-1 ^T are C_16:0 (25.5%), C_17:0 cyclo (14.0%), C_19:0 cyclo ω8c (18.7%), and summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c, 18.1%). The polar lipids of strain QX-1 ^T are mainly diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipid, unidentified aminophospholipid, and five unidentified lipids. The main respiratory quinone is Q-9. The G + C content of its chromosomal DNA is 54.4 mol%. Its fatty acid profile, respiratory quinones, and G + C content also support the placement of QX-1 ^T in the genus Halomonas. These phylogenetic, phenotypic, and chemotaxonomic analyses indicate that QX-1 ^T is a novel species, for which the name Halomonas maris is proposed. The type strain is QX-1 ^T (= MCCC 1A17875^T = KCTC 82198 ^T = NBRC 114670 ^T).

Keywords: Genomic analysis; Halomonas maris sp. nov.; Novel species; Salt tolerance.