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. 1988 May;8(5):2237-41.
doi: 10.1128/mcb.8.5.2237-2241.1988.

Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities

M S Swanson  1 , G Dreyfuss
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Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities

M S Swanson et al. Mol Cell Biol. 1988 May.

Abstract

Several proteins of heterogeneous nuclear ribonucleoprotein (hnRNP) particles display very high binding affinities for different ribonucleotide homopolymers. The specificity of some of these proteins at high salt concentrations and in the presence of heparin allows for their rapid one-step purification from HeLa nucleoplasm. We show that the hnRNP C proteins are poly(U)-binding proteins and compare their specificity to that of the previously described cytoplasmic poly(A)-binding protein. These findings provide a useful tool for the classification and purification of hnRNP proteins from various tissues and organisms and indicate that different hnRNP proteins have different RNA-binding specificities.

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References

    1. Proc Natl Acad Sci U S A. 1973 Mar;70(3):924-8 - PubMed
    1. Genes Dev. 1988 Feb;2(2):215-27 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 May;71(5):1882-6 - PubMed
    1. J Biol Chem. 1975 Aug 10;250(15):6160-7 - PubMed
    1. Eur J Biochem. 1975 Aug 15;56(2):335-41 - PubMed

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