Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jun;26(2):113-120.
doi: 10.1007/s10911-021-09484-5. Epub 2021 Apr 17.

A Method to Pre-Screen Rat Mammary Gland Whole-Mounts Prior To RNAscope

Emily L Duderstadt  1 , Mary Ann Sanders  2 , David J Samuelson  3   4
Affiliations

A Method to Pre-Screen Rat Mammary Gland Whole-Mounts Prior To RNAscope

Emily L Duderstadt et al. J Mammary Gland Biol Neoplasia. 2021 Jun.

Abstract

RNAscope is a quantitative in situ gene expression measurement technique that preserves the spatial aspect of intact tissue; thus, allowing for comparison of specific cell populations and morphologies. Reliable and accurate measurement of gene expression in tissue is dependent on preserving RNA integrity and the quantitative nature of RNAscope. The purpose of this study was to determine if the quantitative nature of RNAscope was retained following processing and carmine staining of mammary gland whole-mounts, which are commonly used to identify lesions, such as hyperplasia and ductal carcinoma in situ (DCIS). We were concerned that handling and procedures required to visualize microscopic disease lesions might compromise RNA integrity and the robustness of RNAscope. No effect on the quantitative abilities of RNAscope was detected when mammary gland whole-mounts were pre-screened for lesions of interest prior to RNAscope. This was determined in comparison to tissue that had been formalin-fixed and paraffin embedded (FFPE) immediately after collection. The ability to pre-screen whole-mounts allowed unpalpable diseased lesions to be identified without labor-intensive serial sectioning of tissue samples to find diseased tissue. This method is applicable to evaluate mammary gland whole-mounts during normal mammary gland development, function, and disease progression.

Keywords: Mammary gland whole-mount tissue screening; Quantitative biology; RNAscope.

PubMed Disclaimer

References

    1. Burska AN, et al. Gene expression analysis in RA: towards personalized medicine. Pharmacogenomics J. 2014;14(2):93–106. - DOI
    1. Patsialou A, et al. Selective gene-expression profiling of migratory tumor cells in vivo predicts clinical outcome in breast cancer patients. Breast cancer research : BCR. 2012;14(5):R139–R139. - DOI
    1. van 't Veer, L.J., et al., Gene expression profiling predicts clinical outcome of breast cancer. Nature, 2002. 415(6871): p. 530–6.
    1. Hamburg MA, Collins FS. The path to personalized medicine. N Engl J Med. 2010;363(4):301–4. - DOI
    1. Wang F, et al. RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. The Journal of molecular diagnostics : JMD. 2012;14(1):22–9. - DOI

Publication types

MeSH terms

LinkOut - more resources