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. 2021 Nov 10;37(11):1505-1509.
doi: 10.2116/analsci.21P068. Epub 2021 Apr 16.

Visualization of the Orientation of Amphipathic Peptides at the Air-Water Interface by X-Ray Reflectometry

Mari Okuno  1 , Makoto Itagaki  2 , Taka-Aki Ishibashi  2 , Yohko F Yano  3
Affiliations
Free article

Visualization of the Orientation of Amphipathic Peptides at the Air-Water Interface by X-Ray Reflectometry

Mari Okuno et al. Anal Sci. .
Free article

Abstract

X-ray reflectivity measurements were performed for the leucine and lysine-based LKα14 peptide designed to adopt an α-helical conformation at the air-water interface. The electron density profiles along the surface normal were calculated from the atomic coordinates predicted by an electronic structure program to fit the X-ray reflectivity curve. At the concentration of the monolayer formation, the long axis of the α-helix adsorbed parallel to the water surface, and the central part was revealed to be submerged in water. On the other hand, at 100 times higher than the surface area density of the monolayer formation, the double layer is formed in which the long axis of the α-helix is parallel to the water surface and only the peptide in the second layer is submerged in water.

Keywords: LKα14 peptide; X-ray reflectometry; air–water interface; electron density profiles; monolayer.

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