Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar 29:8:633442.
doi: 10.3389/fmed.2021.633442. eCollection 2021.

Serum 8-iso-PGF2α Predicts the Severity and Prognosis in Patients With Community-Acquired Pneumonia: A Retrospective Cohort Study

Ling Zheng  1 , Jun Fei  1 , Chun-Mei Feng  1 , Zheng Xu  1 , Lin Fu  1   2 , Hui Zhao  1
Affiliations

Serum 8-iso-PGF2α Predicts the Severity and Prognosis in Patients With Community-Acquired Pneumonia: A Retrospective Cohort Study

Ling Zheng et al. Front Med (Lausanne). .

Abstract

Background: Many studies have identified the important role of 8-isoprostane (8-iso-PGF2α) in pulmonary diseases. However, the role of 8-iso-PGF2α in community-acquired pneumonia (CAP) remains unclear. Therefore, the main goal was to investigate the correlations of serum 8-iso-PGF2α with the severity and prognosis in CAP patients through a hospital-based retrospective cohort study. Methods: All 220 patients with CAP were enrolled. Demographic information and clinical data were collected. Levels of 8-iso-PGF2α and inflammatory cytokines were detected in serum using ELISA. Results: The levels of 8-iso-PGF2α were gradually increased in parallel with the CAP severity scores. Univariate and multivariate logistic regression analyses revealed a positive association between serum 8-iso-PGF2α and the CAP severity scores. Additionally, serum 8-iso-PGF2α levels were positively correlated with circulating inflammatory cytokines (CRP and TNFα). Serum 8-iso-PGF2α levels were increased in the patients with a longer hospital stay than those with a shorter hospital stay. Additionally, 20 patients died after hospitalization. Dead patients presented a higher serum 8-iso-PGF2α than surviving patients. A subsequent survival analysis revealed that higher serum 8-iso-PGF2α levels positively correlated with the risk of death in patients with CAP. Conclusions: Serum 8-iso-PGF2α levels on admission are positively associated with the severity of CAP patients. Elevated serum 8-iso-PGF2α on admission prolongs hospital stay and increases the risk of death in patients with CAP, indicating that 8-iso-PGF2α may be involved in the progression of CAP and serve as an early serum prognostic biomarker for CAP.

Keywords: 8-iso-PGF2α; CAP; CAP severity scores; biomarker; inflammatory cytokines.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Flow diagram of recruitment and follow-up research in this cohort study.
Figure 2
Figure 2
The levels of serum 8-iso-PGF2α in patients with CAP and Control subjects. (A–F) Serum 8-iso-PGF2α was measured using ELISA in CAP patients and Control subjects. (A) The levels of serum 8-iso-PGF2α were compared in patients with CAP and Control subjects. (B) The levels of serum 8-iso-PGF2α according to different grades of the CURB-65 score in patients with CAP. (C) The levels of serum 8-iso-PGF2α in different grades of CRB-65 score in patients with CAP. (D) The levels of serum 8-iso-PGF2α in different grades of the CURXO score in patients with CAP. (E) The levels of serum 8-iso-PGF2α in different grades of PSI score in patients with CAP. (F) The levels of serum 8-iso-PGF2α in different grades of SMART-COP score in patients with CAP. All data were expressed as mean ± SEM. *P < 0.05, **P < 0.01.
Figure 3
Figure 3
The levels of serum 8-iso-PGF2α in alive and dead CAP patients. (A,B) Serum 8-iso-PGF2α was measured using ELISA. (A) The levels of serum 8-iso-PGF2α in CAP patients with different lengths of hospital stay. (B) The levels of serum 8-iso-PGF2α in alive and dead patients. (C) Kaplan Meier plot of the probability survival of CAP patients during hospitalization. All data were expressed as mean ± SEM. *P < 0.05, **P < 0.01.
See this image and copyright information in PMC

References

    1. Franco J. Community-acquired pneumonia. Radiol Technol. (2017) 88:621–36. 10.7861/clinmedicine.12-6-538 - DOI - PubMed
    1. Musher DM, Thorner AR. Community-acquired pneumonia. N Engl J Med. (2014) 371:1619–28. 10.1056/NEJMra1312885 - DOI - PubMed
    1. Lanks CW, Musani AI, Hsia DW. Community-acquired pneumonia and hospital-acquired pneumonia. Med Clin North Am. (2019) 103:487–501. 10.1016/j.mcna.2018.12.008 - DOI - PubMed
    1. Waterer GW. Community-acquired pneumonia: a global perspective. Semin Respir Crit Care Med. (2016) 37:799–805. 10.1055/s-0036-1592313 - DOI - PubMed
    1. Niederman MS. Community-acquired pneumonia: the U.S. perspective. Semin Respir Crit Care Med. (2009) 30:179–88. 10.1055/s-0029-1202937 - DOI - PubMed