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. 2021 Apr 8;7(4):e06734.
doi: 10.1016/j.heliyon.2021.e06734. eCollection 2021 Apr.

Antioxidant activity of polyphenolic compounds obtained from Euphorbia antisyphilitica by-products

Israel Bautista-Hernández  1 , Néstor E Aranda-Ledesma  1 , Romeo Rojas  1 , Julio C Tafolla-Arellano  2 , Guillermo C G Martínez-Ávila  1
Affiliations

Antioxidant activity of polyphenolic compounds obtained from Euphorbia antisyphilitica by-products

Israel Bautista-Hernández et al. Heliyon. .

Abstract

In this study, an alternative extraction technique, ultrasound-assisted extraction, was used to extract the polyphenolic fraction from two different residues of the candelilla plant (Euphorbia antisyphilitica). These metabolites were further analyzed to evaluate their bioactivity as antioxidants. In addition, their functional groups were identified by Fourier transform infrared (FTIR) spectroscopy. The antioxidant assays showed statistically significant differences between the phenolic extracts, with citric acid residues (CAR) exhibiting a higher oxidant effect than sulfuric acid residues (SAR). The CAR from San Jerónimo (SJ) cultivar showed decreased IC50 values (179.441 ± 7.92 μL mL-1, DPPH•), and its polyphenolic fraction was able to inhibit lipid oxidation (70.31 ± 2.50%). FTIR analysis subsequently revealed the presence of functional groups related to polyphenolic compounds, such as hydroxy, carbonyl, carbon double bond, and amine groups. In addition, FTIR spectra showed slight differences in phenolic compounds, due to the strong acid treatment involved in the extraction of wax. The present study demonstrated that candelilla by-products from citric acid-wax extraction have a polyphenolic fraction with strong antioxidant activity, which may be useful in food and pharmaceutical products.

Keywords: Antioxidant activity; E. antisyphilitica Zucc.; Polyphenolic compounds; Ultrasound-assisted extraction.

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Conflict of interest statement

The authors declare the following conflict of interests: Guillermo C. G. Martínez-Ávila; [is part of the Advisory board of the Food Science and Nutrition section of Heliyon].

Figures

Figure 1
Figure 1
General process diagram of antioxidant activity evaluation of E. antisyphilitica by-products.
Figure 2
Figure 2
IR spectral data of polyphenolic compounds of E. antisyphilitica by-products from different wax extraction methods. IR spectra obtained by sulfuric acid method (a); IR spectra obtained by citric acid method (b).
See this image and copyright information in PMC

References

    1. Lan Y. Plant wax. In: Melton L., Shahidi F., Varelis P., editors. Encyclopedia of Food Chemistry. Vol. 1. Elsevier; 2019. pp. 154–196.
    1. Anda J., Shear H. Potential of vertical hydroponic agriculture in Mexico. Sustainability. 2017;9:140.
    1. Granados R., Soria J., Cortina M. Rainfall variability, rainfed agriculture and degree of human marginality in North Guanajuato, Mexico. Singapore J. Trop. Geogr. 2017;38:153–166.
    1. Govea M., La Candelilla . Vol. 52. Secretaría de Medio Ambiente; 2018. La última esperanza de algunos pueblos del desierto.
    1. Rojas R., Saucedo S., De León M.A., Jasso D., Aguilar C.N. Essay past, present and future of candelilla. Rev. Mex. Cienc. Agric. 2011;2(6):7–18. ISSN: 2007-1132.

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