MAP kinase signal transduction pathways in plants

Abstract

The mitogen-activated protein kinase (MAP kinase) signal transduction cascades are routes through which eukaryotic cells deliver extracellular messages to the cytosol and nucleus. These signalling pathways direct cell division, cellular differentiation, metabolism, and both biotic and abiotic stress responses. In plants, MAP kinases and the upstream components of the cascades are represented by multigene families, organized into different pathways which are stimulated and interact in complex ways. Experimental strategies for the analysis of MAP kinase cascades include the yeast two-hybrid system; using this approach in vitro interactions between specific MAP kinase cascade components have been analysed and putative plant cascades postulated. Transient transformation of protoplasts with epitope-tagged kinases has allowed cascades to be tested in planta. There is clear evidence for the involvement of MAP kinases in plant cell division and in the regulation of auxin signalling. Biotic (pathogens and pathogen-derived elicitors from fungi, bacteria and viruses) and abiotic stresses including wounding, mechanical stimulation, cold, drought and ozone can elicit defence responses in plants through MAP kinase pathways. There are data suggesting that ABA signalling utilizes a MAP kinase pathway, and probably ethylene and perhaps cytokinins do so also. The objective of this paper is to review this rapidly advancing field. Contents Summary 67 I. Introduction 68 II. Background 68 III. MAP kinase targets and targeting specificity 69 IV. Assays and inhibitors 70 V. Two well characterized MAP kinase pathways, Hog1 and Sevenless 71 VI. MAP kinases in plants 73 VII. MAP kinases and cell division 76 VIII. MAP kinases and plant hormones 76 IX. MAP kinase and abiotic stress 78 X. MAP kinase and biotic stress 80 XI. Future perspectives for MAP kinase research in plants 83 Acknowledgements 84 References 84.

Keywords: MAP kinase; MEK; plant hormone signalling; plant stress signalling; protein phosphorylation; signal transduction.