Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 May;154(2):451-460.
doi: 10.1046/j.1469-8137.2002.00376.x.

Glutathione status correlates with different degrees of desiccation tolerance in three lichens

Ilse Kranner  1
Affiliations
Free article

Glutathione status correlates with different degrees of desiccation tolerance in three lichens

Ilse Kranner. New Phytol. 2002 May.
Free article

Abstract

• Glutathione-related mechanisms of coping with desiccation were studied in three lichens (Pseudevernia furfuracea, Peltigera polydactyla and Lobaria pulmonaria) with different degrees of tolerance. Reduced (GSH) and oxidised glutathione (GSSG), glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PDH) were measured during short- and long-term desiccation, and subsequent rehydration. • Glutathione was analysed by HPLC, and enzymes by spectrophotometry. Lichens were desiccated over silica gel, and rehydrated either in liquid water or water vapour. • Desiccation caused oxidation of almost all of the GSH. After short-term desiccation, rehydration caused rapid reduction of GSSG in all three species. Following long-term desiccation, P. furfuracea (the most desiccation-tolerant lichen studied) rapidly increased GSH when rehydrated in liquid water or water vapour. However, in P. polydactyla, neither method of rehydration re-established the initial GSH pool. After long-term desiccation, L. pulmonaria regenerated initial concentrations of GSH only when rehydrated in liquid water. • Reduction of GSSG was correlated with re-establishment of the predesiccation activity of G6PDH, a key enzyme of the oxidative pentose shunt. This enzyme probably provides NADPH during the first stages of rehydration, when photosynthesis is not yet possible.

Keywords: antioxidants; desiccation tolerance; glutathione; lichens.

PubMed Disclaimer

References

    1. Bartosz G. 1997. Oxidative stress in plants. Acta Physiologiae Plantarum 154: 47 - 64.
    1. Beckett RP. 1995. Some aspects of the water relations of lichens studies using thermocouple psychrometry. Annals of Botany 76: 211 - 217.
    1. Bergmann L, Rennenberg H. 1993. Glutathione metabolism in plants. In: DeKok LJ, Stulen I, Rennenberg H, Brunold C, Rauser WE, eds. Sulphur nutrition and assimilation in higher plants. Regulatory agricultural and environmental aspects. The Hague, The Netherlands: SPB Academic Publishing, 109 - 123.
    1. Bernacchia G, Salamini F, Bartels D. 1996. Molecular characterization of the rehydration process in the resurrection plant Craterostigma plantagineum. Plant Physiology 111: 1043 - 1050.
    1. Bewley DJ. 1979. Physiological aspects of desiccation tolerance. Annual Reviews of Plant Physiology 30: 195 - 238.

LinkOut - more resources