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. 2004 Aug;163(2):325-331.
doi: 10.1111/j.1469-8137.2004.01098.x.

Temperature responses of growth, photosynthesis, respiration and NADH: nitrate reductase in cryophilic and mesophilic algae

Vincenza Vona  1 , Vittoria Di Martino Rigano  2 , Ornella Lobosco  1 , Simona Carfagna  1 , Sergio Esposito  1 , Carmelo Rigano  1
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Free article

Temperature responses of growth, photosynthesis, respiration and NADH: nitrate reductase in cryophilic and mesophilic algae

Vincenza Vona et al. New Phytol. 2004 Aug.
Free article

Abstract

• Temperature effects on growth, photosynthesis, respiration and nitrate reductase (NR) were studied in the cryophilic algae Koliella antarctica and 'Chlorella'saccharophila, and in the mesophilic Chlorella sorokiniana. • Growth rate was measured as increase in optical density. Photosynthesis at saturating light and respiration in darkness were measured as O2 exchange. NADH : NR was assayed in crude extracts. • The two cryophilic algae grew below 15°C, and C. sorokiniana above 20°C. Photosynthetic and respiration rates of K. antarctica and 'C.' saccharophila were elevated at 5°C, and peaked at 30°C. Arrhenius plots from 5 to 25°C were linear in K. antarctica, whereas in 'C.' saccharophila and C. sorokiniana they exhibited breaks at 15 and 20°C, respectively. Values for activation energy (Ea ) and the factor by which the rate increases with raising the temperature 10°C (Q10 ) differed. Nitrate reductase had its optimum at 25°C in cryophilic algae and at 35°C in C. sorokiniana. • We conclude that growth of cryophilic algae at low temperature is favoured by elevated photosynthesis and respiration rates, but that it could be limited by a high respiration : photosynthesis ratio.

Keywords: algal growth; algal photosynthesis; algal respiration; cryophilic algae; nitrate reductase; temperature dependence.

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