Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Dec;104(4):631-636.
doi: 10.1111/j.1469-8137.1986.tb00663.x.

THE EFFECT OF A PHYTOTOXIN FROM ALTERNARIA BRASSICICOLA ON BRASSICA POLLEN

T Hodgkin  1 , M V Macdonald  2
Affiliations

THE EFFECT OF A PHYTOTOXIN FROM ALTERNARIA BRASSICICOLA ON BRASSICA POLLEN

T Hodgkin et al. New Phytol. 1986 Dec.

Abstract

A methanol-soluble extract from the culture filtrate of Alternaria brassicicola (Schw.) Wilts., grey leaf spot, markedly inhibited pollen germination and pollen tube growth of Brassica napus L., Brassica campestris L. and Brassica oleracea L. in hanging drop culture at concentrations comparable to those causing death of B. napus secondary embryoids. The extract was partially purified by Sephadex column chromatography, and the fractions obtained were tested for their inhibitory capacity. Two fractions which killed secondary embryoids also inhibited pollen germination. The methanol-soluble extract and inhibitory fractions from Sephadex chromatography were further tested using a thin-layer chromatographic bioassay which showed that the original extract could be separated into at least four inhibitory compounds. The potential of the techniques used for screening and selection for resistance to compounds toxic to plants is briefly outlined.

Keywords: Alternaria brassicicola; Brassica spp.; bioassay; fungal toxin; pollen.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Behnke, M. (1979). Selection of potato callus for resistance to culture filtrates of Phytophtora infestans and regeneration of resistant plants. Theoretical and Applied Genetics, 55, 69-71.
    1. Brettell, R., Ingram, D. S. & Thomas, E. (1980). Selection of maize tissue cultures resistant to Dreschlera (Hetminthosporium) maydis T-toxin. In: Tissue Culture Methods for Plant Pathologists (Ed. By D. S. Ingram & J. P. Helgeson), pp. 223-231. Blackwell, Oxford .
    1. Darlington, C. D. & Lacour, L. F. (1960). The Handling of Chromosomes, 3rd Edn. Allen & Unwin, London .
    1. Hodgkin, X. (1983). A medium for germinating brassica pollen in vivo. Cruciferae Newsletter, 8, 63.
    1. Hodgkin, T. & Lyon, G. D. (1986). The effect of Brassica oleracea stigma extracts on the germination of B. oleracea pollen in a thin layer chromatographic bioassay. Journal of Experimental Botany, 37, 406-411.

LinkOut - more resources