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. 1988 Feb;108(2):211-218.
doi: 10.1111/j.1469-8137.1988.tb03698.x.

Early events of vesicular-arbuscular mycorrhiza formation on Ri T-DNA transformed roots

G Bécard  1 , J A Fortin  1
Affiliations

Early events of vesicular-arbuscular mycorrhiza formation on Ri T-DNA transformed roots

G Bécard et al. New Phytol. 1988 Feb.

Abstract

An in vitro system using Ri T-DXA transformed roots and the vesicular-arbuscular mycorrhizal fungus Gigaspora margarita Becker & Hall has been developed to study the initial events of mycorrhiza formation. Sucrose, sodium and phosphorus were found to be critical components of the medium used to establish the dual culture. Using a single spore as inoculum it was consistently possible to obtain colonization of a preselected point on the root and to time the colonization process (within 5 days). Abundant viable and aseptic spores can be obtained. The system is especially appropriate for studying the triggering of the fungal biotrophy towards the root.

Keywords: Key words: Transformed roots, Gigaspora margarita, in vitro endomycorrhizas, sporulation.

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References

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