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. 2021 May;70(5):605-618.
doi: 10.1007/s00011-021-01462-1. Epub 2021 Apr 20.

Glycine is a competitive antagonist of the TNF receptor mediating the expression of inflammatory cytokines in 3T3-L1 adipocytes

Rodrigo Romero-Nava  1   2   3 , Francisco J Alarcón-Aguilar  1 , Abraham Giacoman-Martínez  1 , Gerardo Blancas-Flores  1 , Karla A Aguayo-Cerón  3 , Martha A Ballinas-Verdugo  4 , Fausto Sánchez-Muñoz  4 , Fengyang Huang  2 , Santiago Villafaña-Rauda  3 , Julio C Almanza-Pérez  5
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Glycine is a competitive antagonist of the TNF receptor mediating the expression of inflammatory cytokines in 3T3-L1 adipocytes

Rodrigo Romero-Nava et al. Inflamm Res. 2021 May.

Abstract

Objective: To determine the involvement of TNF-α and glycine receptors in the inhibition of pro-inflammatory adipokines in 3T3-L1 cells.

Methods: RT-PCR evidenced glycine receptors in 3T3-L1 adipocytes. 3T3-L1 cells were transfected with siRNA for the glycine (Glrb) and TNF1a (Tnfrsf1a) receptors and confirmed by confocal microscopy. Transfected cells were treated with glycine (10 mM). The expressions of TNF-α and IL-6 mRNA were measured by qRT-PCR, while concentrations were quantified by ELISA.

Results: Glycine decreased the expression and concentration of TNF-α and IL-6; this effect did not occur in the absence of TNF-α receptor due to siRNA. In contrast, glycine produced only slight changes in the expression of TNF-α and IL-6 in the absence of the glycine receptor due to siRNA. A docking analysis confirmed the possibility of binding glycine to the TNF-α1a receptor.

Conclusion: These findings support the idea that glycine could partially inhibit the binding of TNF-α to its receptor and provide clues about the mechanisms by which glycine inhibits the secretion of pro-inflammatory adipokines in adipocytes through the TNF-α receptor.

Keywords: Adipokines; Glycine receptor; Inflammation; TNF-α receptor; siRNA.

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References

    1. Alarcon-Aguilar FJ, Almanza-Perez J, Blancas G, Angeles S, Garcia-Macedo R, Roman R, Cruz M. Glycine regulates the production of pro-inflammatory cytokines in lean and monosodium glutamate-obese mice. Eur J Pharmacol. 2008;599:152–8. - PubMed
    1. Almanza-Perez JC, Alarcon-Aguilar FJ, Blancas-Flores G, Campos-Sepulveda AE, Roman-Ramos R, Garcia-Macedo R, Cruz M. Glycine regulates inflammatory markers modifying the energetic balance through PPAR and UCP-2. Biomed Pharmacother. 2010;64(8):534–40. - PubMed
    1. Bahmani F, Bathaie SZ, Aldavood SJ, Ghahghaei A. Glycine therapy inhibits the progression of cataract in streptozotocin-induced diabetic rats. Mol Vis. 2012;18:439. - PubMed - PMC
    1. Barnes PJ. Nuclear factor-kappa B. Int J Biochem Cell Biol. 1997;29(6):867–70. - PubMed
    1. Barnes PJ, Karin M. Nuclear factor-kappa B: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med. 1997;336(15):1066–71. - PubMed

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