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. 2021 May;70(5):605-618.
doi: 10.1007/s00011-021-01462-1. Epub 2021 Apr 20.

Glycine is a competitive antagonist of the TNF receptor mediating the expression of inflammatory cytokines in 3T3-L1 adipocytes

Rodrigo Romero-Nava  1   2   3 , Francisco J Alarcón-Aguilar  1 , Abraham Giacoman-Martínez  1 , Gerardo Blancas-Flores  1 , Karla A Aguayo-Cerón  3 , Martha A Ballinas-Verdugo  4 , Fausto Sánchez-Muñoz  4 , Fengyang Huang  2 , Santiago Villafaña-Rauda  3 , Julio C Almanza-Pérez  5
Affiliations

Glycine is a competitive antagonist of the TNF receptor mediating the expression of inflammatory cytokines in 3T3-L1 adipocytes

Rodrigo Romero-Nava et al. Inflamm Res. 2021 May.

Abstract

Objective: To determine the involvement of TNF-α and glycine receptors in the inhibition of pro-inflammatory adipokines in 3T3-L1 cells.

Methods: RT-PCR evidenced glycine receptors in 3T3-L1 adipocytes. 3T3-L1 cells were transfected with siRNA for the glycine (Glrb) and TNF1a (Tnfrsf1a) receptors and confirmed by confocal microscopy. Transfected cells were treated with glycine (10 mM). The expressions of TNF-α and IL-6 mRNA were measured by qRT-PCR, while concentrations were quantified by ELISA.

Results: Glycine decreased the expression and concentration of TNF-α and IL-6; this effect did not occur in the absence of TNF-α receptor due to siRNA. In contrast, glycine produced only slight changes in the expression of TNF-α and IL-6 in the absence of the glycine receptor due to siRNA. A docking analysis confirmed the possibility of binding glycine to the TNF-α1a receptor.

Conclusion: These findings support the idea that glycine could partially inhibit the binding of TNF-α to its receptor and provide clues about the mechanisms by which glycine inhibits the secretion of pro-inflammatory adipokines in adipocytes through the TNF-α receptor.

Keywords: Adipokines; Glycine receptor; Inflammation; TNF-α receptor; siRNA.

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