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. 2021 Nov 28;31(11):1545-1551.
doi: 10.4014/jmb.2101.01026.

Overexpression of Capsular Polysaccharide Biosynthesis Protein in Lactobacillus plantarum P1 to Enhance Capsular Polysaccharide Production for Di-n-butyl Phthalate Adsorption

Wei-Bing Liu  1 , Zhi-Wei Lin  1 , Ying Zhou  1 , Bang-Ce Ye  1
Affiliations

Overexpression of Capsular Polysaccharide Biosynthesis Protein in Lactobacillus plantarum P1 to Enhance Capsular Polysaccharide Production for Di-n-butyl Phthalate Adsorption

Wei-Bing Liu et al. J Microbiol Biotechnol. .

Abstract

Exopolysaccharides (EPSs) such as capsular polysaccharide (CPS) are important bioactive carbohydrate compounds and are often used as bioenrichment agents and bioabsorbers to remove environmental pollutants like di-n-butyl phthalate (DBP). Among the EPS-producing bacteria, lactic acid bacteria (LAB) have gained the most attention. As generally recognized as safe (GRAS) microorganisms, LAB can produce EPSs having many different structures and no health risks. However, EPS production by LAB does not meet the needs of large-scale application on an industrial scale. Here, the capA gene (encoding CPS biosynthesis protein) was overexpressed in Lactobacillus plantarum P1 to improve the production of EPSs and further enhance the DBP adsorption capability. Compared with P1, the CPS production in capA overexpressed strain was increased by 11.3 mg/l, and the EPS thickness was increased from 0.0786 ± 0.0224 μm in P1 to 0.1160 ± 0.0480 μm in P1-capA. These increases caused the DBP adsorption ratio of P1-capA to be doubled. Overall, the findings in this study provide a safe method for the adsorption and removal of DBP.

Keywords: Lactobacillus plantarum; adsorption; di-n-butyl phthalate; exopolysaccharides; overexpression; probiotics.

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Figures

Fig. 1
Fig. 1
The biosynthetic pathway of CPS.
Fig. 2
Fig. 2. Validation of capA overexpression.
(A) Western blot analysis of CapA in recombinant bacteria; P1 without the plasmid and P1 with the original plasmid pMG36e were used as controls. (B) Analysis of capA gene overexpression at transcriptional level.
Fig. 3
Fig. 3
Evaluation of strain growth and CPS production in P1 (A) and P1-capA (B).
Fig. 4
Fig. 4. Transmission electron microscopy for illustration of EPSs in P1 (A) and P1-capA (B).
The numbers represent the thickness (micrometer) of capsular polysaccharide in P1 (A) and P1-capA (B).
Fig. 5
Fig. 5. DBP adsorption capacity of P1 and P1-capA.
The experiments were duplicated three times and the data were presented with standard deviation. *p < 0.05.
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