Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar 3;53(2):364-370.
doi: 10.19723/j.issn.1671-167X.2021.02.022.

[Efficacy of two barrier membranes and deproteinized bovine bone mineral on bone regeneration in extraction sockets: A microcomputed tomographic study in dogs]

[Article in Chinese]
S W Wang  1 , P Y You  1 , Y H Liu  1 , X Z Wang  1 , L Tang  1 , M Wang  1
Affiliations

[Efficacy of two barrier membranes and deproteinized bovine bone mineral on bone regeneration in extraction sockets: A microcomputed tomographic study in dogs]

[Article in Chinese]
S W Wang et al. Beijing Da Xue Xue Bao Yi Xue Ban. .

Abstract
in English, Chinese

Objective: To evaluate the effect of two barrier membranes [multilaminated small intestinal submucosa (mSIS) and bioresorable collagen membrane (Bio-Gide)] combined with deproteinized bovine bone mineral Bio-Oss on guided bone regeneration through a canine extraction sockets model.

Methods: The distal roots of 18 premolars of the Beagle' s bilateral maxillary and mandibular were removed, and 18 extraction sockets were obtained. They were randomly divided into 3 groups, and the following procedures were performed on the sockets: (1) filled with Bio-Oss and covered by mSIS (mSIS group), (2) filled with Bio-Oss and covered by Bio-Gide (BG group), (3) natural healing (blank control group). Micro-computed tomograph (Micro-CT) was performed 4 and 12 weeks after surgery to eva-luate the new bone regeneration in the sockets of each group.

Results: The postoperative healing was uneventful in all the animals, and no complications were observed through the whole study period. Micro-CT analysis showed that the new bone fraction in the mSIS group and the BG group was significantly higher than that in the blank control group at the end of 4 weeks and 12 weeks (P < 0.05), and more new bone fraction was observed in the mSIS group than in the BG group, but the difference was not statistically significant (P>0.05). The new bone fraction of coronal third part of the socket in the mSIS group and BG group at the end of 4 weeks were significantly higher than that of the middle and apical third part of each group (P < 0.05). The values of bone mineral density were similar at 4 weeks in all the groups (P>0.05), but were significantly higher than that in the control group at the end of 12 weeks (P < 0.05). The bone morphometric analysis showed that the trabecular number and trabecular spacing were significantly better in the mSIS group and the BG group than in the control group at the end of 4 weeks and 12 weeks (P < 0.05), while the value in the mSIS group was slightly higher than in the BG group, but the difference was not statistically significant (P>0.05). The difference in trabecular thickness between all the groups was not statistically significant (P>0.05).

Conclusion: mSIS membrane as a barrier membrane combined with deproteinized bovine bone mineral can enhance new bone formation in canine extraction sockets, similar to Bio-Gide collagen membrane.

目的: 建立犬拔牙窝模型，采用影像学分析方法评价拔牙窝内植入去蛋白牛骨基质骨粉颗粒Bio-OssⓇ(简称Bio-Oss骨粉)并覆盖复层猪小肠黏膜下层膜(multilaminated small intestinal submucosa membrane, mSIS)或可吸收胶原膜Bio-GideⓇ(简称Bio-Gide膜)，愈合4周和12周后的牙槽窝内成骨效果。

方法: 拔除3只比格犬双侧上下颌共计18颗前磨牙的远中根，得到18个拔牙窝，随机平均分为3大组，并分别对各拔牙窝组进行以下操作：(1)植入Bio-Oss骨粉并覆盖mSIS膜(mSIS组)，(2)植入Bio-Oss骨粉并覆盖Bio-Gide膜(BG组)，(3)自然愈合(空白对照组)。每大组各随机平均分为2个小组，分别于手术后4周和12周取样进行微计算机体层扫描(micro-computed tomograph, Micro-CT)，检测评价各组牙槽窝内新骨的生长情况，比较mSIS膜和Bio-Gide膜对拔牙窝内骨再生的影响。

结果: Micro-CT分析显示，mSIS组和BG组在术后4周和12周的新生骨容积比均显著高于空白对照组(P < 0.05)，其中mSIS组略高于BG组，但两组间差异无统计学意义(P>0.05)。术后4周mSIS组和BG组的牙槽窝冠1/3区域新生骨容积比例显著高于中1/3及根1/3区域(P < 0.05)。术后4周各组的新生骨密度值相近(P>0.05)，术后12周时mSIS组和BG组的新生骨密度值均显著高于对照组(P < 0.05)。术后4周和12周mSIS组和BG组的新生骨小梁的数量以及排列紧凑程度明显优于空白对照组(P < 0.05)，而mSIS略优于BG组，但两组间差异无统计学意义(P>0.05)。各组间骨小梁厚度的差异无统计学意义(P>0.05)。

结论: 两种屏障膜联合去蛋白牛骨基质植入拔牙窝内有利于新骨再生，mSIS膜与Bio-Gide膜的应用效果相似。

Keywords: Alveolar bone loss; Guided bone regeneration; Membranes; Tooth extraction.

PubMed Disclaimer

Figures

图 1
图 1
各组拔牙窝的处理方法 Schematic representation of the treatment of all groups
图 2
图 2
手术步骤 Clinical view of the surgical procedures
图 3
图 3
术后4周牙槽窝新生骨Micro-CT图像(黄色箭头示新生骨，绿色箭头示骨移植材料) Micro-CT images at 4 weeks of healing showing new bone (yellow arrows) and bone substitute materials (green arrows)
图 4
图 4
术后12周牙槽窝新生骨Micro-CT图像(黄色箭头示新生骨，绿色箭头示骨移植材料) Micro-CT images at 12 weeks of healing showing new bone (yellow arrows) and bone substitute materials (green arrows)
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Ersanli S, Olgac V, Leblebicioglu B. Histologic analysis of alveolar bone following guided bone regeneration. J Periodontol. 2004;75(5):750–756. doi: 10.1902/jop.2004.75.5.750. - DOI - PubMed
    1. Chiapasco M, Zaniboni M. Clinical outcomes of GBR procedures to correct peri-implant dehiscences and fenestrations: a systematic review. Clin Oral Implants Res. 2009;20(Suppl 4):113–123. - PubMed
    1. Oikarinen KS, Sandor GK, Kainulainen VT, et al. Augmentation of the narrow traumatized anterior alveolar ridge to facilitate dental implant placement. Dent Traumatol. 2003;19(1):19–29. doi: 10.1034/j.1600-9657.2003.00125.x. - DOI - PubMed
    1. Amler MH. The time sequence of tissue regeneration in human extraction wounds. Oral Surg Oral Med Oral Pathol. 1969;27(3):309–318. doi: 10.1016/0030-4220(69)90357-0. - DOI - PubMed
    1. Nyman S, Lang NP, Buser D, et al. Bone regeneration adjacent to titanium dental implants using guided tissue regeneration: a report of two cases. Int J Oral Maxillofac Implants. 1990;5(1):9–14. - PubMed