Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jun;21(6):592.
doi: 10.3892/etm.2021.10024. Epub 2021 Apr 8.

Downregulation of miR-588 is associated with tumor progression and unfavorable prognosis in patients with osteosarcoma

Tao Yu  1 , Shuang Liang  2 , Tianhong Ma  2 , Weidong Song  1
Affiliations

Downregulation of miR-588 is associated with tumor progression and unfavorable prognosis in patients with osteosarcoma

Tao Yu et al. Exp Ther Med. 2021 Jun.

Abstract

Osteosarcoma (OS) is a primary malignant tumor characterized by a high metastatic potential and poor prognosis. The dysregulation of miR-588 has been demonstrated to serve crucial roles in the progression of numerous types of cancer. The present study aimed to investigate the expression and function of miR-588 in the development of OS. To do so, clinical samples were collected and analyzed, and in vitro experiments were conducted. A total of 104 patients with OS were recruited between 2012 and 2014. The expression of miR-588 was analyzed by reverse transcription quantitative PCR. The association between miR-588 expression and the clinicopathological characteristics and survival rate of patients with OS was evaluated. Furthermore, Cell Counting Kit-8 and Transwell assays were used to evaluate the effect of miR-588 on the proliferation and the migratory and invasive abilities of various OS cell lines. The results demonstrated that miR-588 expression in OS tissues and cells was significantly lower compared with normal tissues and cells. In addition, miR-588 expression was closely associated with the Musculoskeletal Tumor Society (MSTS) staging of patients with OS. miR-588 expression and MSTS staging were therefore considered as independent indicators for the prognosis of patients with OS. In addition, miR-588 downregulation significantly stimulated the proliferation and migratory and invasive abilities of OS cells. Taken together, these findings indicated that miR-588 may serve as an independent prognostic factor and tumor suppressor in OS.

Keywords: development; miR-588; osteosarcoma; prognosis; tumor progression.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Relative expression of miR-588 in OS tissues and cells. (A) miR-588 was significantly downregulated in OS tissues compared with adjacent normal tissues. ***P<0.001. (B) miR-588 was significantly downregulated in OS cell lines MG63, HKOS-240S, SAOS-2 and U2OS compared with normal cells hFOB1.19. ***P<0.001. OS, osteosarcoma; miR, microRNA.
Figure 2
Figure 2
Kaplan-Meier curve of patients with osteosarcoma according to miR-588 expression levels. Log rank P=0.027. miR, microRNA.
Figure 3
Figure 3
Effects of miR-588 expression level on OS cell proliferation. (A) Cell transfection with miR-588 mimic significantly increased the expression of miR-588 in MG63 and U2OS cells. Transfection of miR-588 inhibitor significantly decreased the expression of miR-588. ***P<0.001 vs. Mock group. (B) Proliferation of MG63 and U2OS cells was promoted by miR-588 knockdown and inhibited miR-588 overexpression. *P<0.05 and **P<0.01 vs. Mock group. OS, osteosarcoma; miR, microRNA; NC, negative control; OD, optical density.
Figure 4
Figure 4
Effects of miR-588 expression level on the migratory and invasive abilities of OS cells. (A) Overexpression of miR-588 significantly decreased the migratory ability of MG63 and U2OS cells. Downregulation of miR-588 significantly increased the migratory ability of MG63 and U2OS cells. ***P<0.001 vs. Mock group. (B) Overexpression of miR-588 significantly inhibited the invasive ability of MG63 and U2OS cells. Downregulation of miR-588 significantly increased the invasive ability of MG63 and U2OS cells. ***P<0.001 vs. Mock group. OS, osteosarcoma; miR, microRNA; NC, negative control.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Picci P. Osteosarcoma (osteogenic sarcoma) Orphanet J Rare Dis. 2007;2(6) doi: 10.1186/1750-1172-2-6. - DOI - PMC - PubMed
    1. Ottaviani G, Jaffe N. The epidemiology of osteosarcoma. Cancer Treat Res. 2009;152:3–13. doi: 10.1007/978-1-4419-0284-9_1. - DOI - PubMed
    1. Liu W, Zhang X, Liu P, Shen X, Lan T, Li W, Jiang Q, Xie X, Huang H. Effects of berberine on matrix accumulation and NF-kappa B signal pathway in alloxan-induced diabetic mice with renal injury. Eur J Pharmacol. 2010;638:150–155. doi: 10.1016/j.ejphar.2010.04.033. - DOI - PubMed
    1. Yoshida K, Okamoto M, Aoki K, Takahashi J, Saito N. A Review of T-Cell Related Therapy for Osteosarcoma. Int J Mol Sci. 2020;21(4877) doi: 10.3390/ijms21144877. - DOI - PMC - PubMed
    1. Punzalan M, Hyden G. The role of physical therapy and occupational therapy in the rehabilitation of pediatric and adolescent patients with osteosarcoma. Cancer Treat Res. 2009;152:367–384. doi: 10.1007/978-1-4419-0284-9_20. - DOI - PubMed