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. 2021 Jul;44(13):2596-2601.
doi: 10.1002/jssc.202100169. Epub 2021 May 25.

Ultra-rapid capillary zone electrophoresis method for simultaneous determination of arginine and ibuprofen

Aliceana Almeida do Prado  1 , Michelle Miranda Araújo Carvalho Ribeiro  1 , Eduardo Mathias Richter  1
Affiliations

Ultra-rapid capillary zone electrophoresis method for simultaneous determination of arginine and ibuprofen

Aliceana Almeida do Prado et al. J Sep Sci. 2021 Jul.

Abstract

The combination of arginine and ibuprofen is widely used for pain relief with a faster onset of action than conventional ibuprofen. Therefore, the determination of both compounds in a single run is highly desirable for rapid quality control applications. This paper reports an ultra-fast method (100 injections/h) for simultaneous determination of arginine and ibuprofen using capillary electrophoresis with capacitively coupled contactless conductivity detection. The separation of arginine as cation and ibuprofen as anion was achieved using a background electrolyte composed by an equimolar mixture of 10 mmol/L of 2-(cyclohexylamino) ethanesulfonic acid and boric acid with pH adjusted to 8.4 using potassium hydroxide. The limits of detections were 5.3 and 10.0 μmol/L for arginine and ibuprofen, respectively. The proposed method is simple, fast (one analysis every 35 s), environmentally friendly (minimal waste generation) and accurate (recovery values between 95 and 98%).

Keywords: contactless detection; environmentally friendly; pharmaceutical analysis; simultaneous quantification; ultrafast analysis.

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References

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