Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Apr 9:9:e11223.
doi: 10.7717/peerj.11223. eCollection 2021.

Optimal extraction, purification and antioxidant activity of total flavonoids from endophytic fungi of Conyza blinii H. Lév

Shuheng Zhao #  1 , Xulong Wu #  2 , Xiaoyu Duan  1 , Caixia Zhou  1 , Zhiqiao Zhao  1 , Hui Chen  1 , Zizhong Tang  1 , Yujun Wan  3 , Yirong Xiao  4 , Hong Chen  5
Affiliations

Optimal extraction, purification and antioxidant activity of total flavonoids from endophytic fungi of Conyza blinii H. Lév

Shuheng Zhao et al. PeerJ. .

Abstract

Background: Flavonoids are widely used in the market because of their antibacterial, antiviral, and antioxidant activities. But the production speed of flavonoids is limited by the growth of plants. CBL9 (Chaetomium cruentum) is a flavonoid-producing endophytic fungi from Conyza blinii H. Lév, which has potential to produce flavonoids.

Methods: In this study, we isolated total flavonoids from endophytic fungus CBL9 of Conyza blinii H. Lév using macroporous resin D101. The process was optimized by response surface and the best extraction process was obtained. The antioxidant activities of total flavonoids were analyzed in vitro.

Results: It was found that the best parameters were 25 °C pH 2.80, 1.85 h, and the adsorption ratio reached (64.14 ± 0.04)%. A total of 60% ethanol was the best elution solvent. The elution ratio of total flavonoid reached to (81.54 ± 0.03)%, and the purity was 7.13%, which was increased by 14.55 times compared with the original fermentation broth. Moreover its purity could rise to 13.69% after precipitated by ethanol, which is very close to 14.10% prepared by ethyl acetate extraction. In the antioxidant research, the clearance ratio of L9F-M on DPPH, ABTS, •OH, •O2-, (96.44 ± 0.04)% and (75.33 ± 0.03)%, (73.79 ± 0.02)%, (31.14 ± 0.01)% at maximum mass concentration, was higher than L9F.

Conclusion: The result indicated using macroporous resin in the extraction of total flavonoid from endophytic fungus is better than organic solvents with higher extraction ratio, safety and lower cost. In vitro testing indicated that the flavonoid extracted by macroporous resin have good antioxidant activity, providing more evidence for the production of flavonoid by biological fermentation method.

Keywords: Chaetomium cruentum; Flavonoid; Macroporous resin; Response surface.

PubMed Disclaimer

Conflict of interest statement

The authors declare there are no competing interests.

Figures

Figure 1
Figure 1. Relationship between adsorption time and adsorption rate.
The data point indicates the change of adsorption rate over time.
Figure 2
Figure 2. Adsorption of D101 under different pH conditions.
The data point indicates the change of adsorption rate over pH.
Figure 3
Figure 3. Adsorption of D101 under different liquid temperature.
The data point indicates the change of adsorption rate over temperature.
Figure 4
Figure 4. Response surface of interrelated influence of temperature and pH to flavonoids rate.
Figure 5
Figure 5. Response surface of interrelated influence of temperature and time to flavonoids rate.
Figure 6
Figure 6. Response surface of interrelated influence of pH and time to flavonoids rate.
Figure 7
Figure 7. Comparison of elution rate and purity of total flavonoids at different concentrations.
The data point indicates the Indicates the change of elution rate and purity of flavonoid over eluent.
Figure 8
Figure 8. Scavenging ability of total flavonoids of endophytic fungi on DPPH.
Each data point indicates the clearance rate of total flavonoids on DPPH before and after extraction, where Vc is the control.
Figure 9
Figure 9. Scavenging ability of total flavonoids of endophytic fungi on ABTS.
Each data point indicates the clearance rate of total flavonoids on ABTS before and after extraction, where Vc is the control.
Figure 10
Figure 10. Scavenging ability of total flavonoids of endophytic fungi on −OH.
Each data point indicates the clearance rate of total flavonoids on −OH before and after extraction, where Vc is the control.
Figure 11
Figure 11. Scavenging ability of total flavonoids of endophytic fungi on •O2−.
Each data point indicates the clearance rate of total flavonoids on •O2− before and after extraction, where Vc is the control.
See this image and copyright information in PMC

References

    1. Aruoma OI, Grootveld M, Bahorun T. Free radicals in biology and medicine: from inflammation to biotechnology. Biofactors. 2006;27(1–4):1–3. doi: 10.1002/biof.5520270101. - DOI - PubMed
    1. Baran MY, Emecen G, Simon A, Tóth G, Kuruuzum-Uz A. Assessment of the antioxidant activity and genotoxicity of the extracts and isolated glycosides with a new flavonoid from Lotus aegaeus (Gris.) Boiss. Industrial Crops and Products. 2020;153:112590–112601. doi: 10.1016/j.indcrop.2020.112590. - DOI
    1. Bi YG, Tan YQ. Study on macroporous resin separation and purification of total flavonoids of plantago process. Advanced Materials Research. 2012;550–553:987–992. doi: 10.4028/www.scientific.net/AMR.550-553.987. - DOI
    1. Carroll GC, Carroll FE. Studies on the incidence of coniferous needle endophytes in the Pacific Northwest. Canadian Journal of Botany. 1978;56(24):3034–3043. doi: 10.1139/b78-367. - DOI
    1. Chen Z, Long J, Kang L, Du X, Di D. Modified macroporous adsorption resin (LX1180) used to adsorb flavonoid. Pigment & Resin Technology. 2013;42(6):374–387. doi: 10.1108/PRT-04-2012-0030. - DOI

LinkOut - more resources