Characterization of the complete chloroplast genome of China Viburnum burejaeticum Regel et Herd and intra-species diversity

Abstract

Viburnum burejaeticum Regel et Herd is widely cultivated in botanical gardens. However, as a member of Adoxaceae, few studies have been carried out on its phylogenetic relationship with other family members. Here we report the first complete chloroplast (cp) genome sequence of V. burejaeticum collected from China. The circular cp genome is 158,381 bp in size, including a large single-copy (LSC) region of 87,067 bp and a small single-copy (SSC) region of 18,212 bp, which were separated by two inverted repeat (IR) regions (26,551 bp each). A total of 126 genes were annotated, including 8 ribosomal RNAs (rRNAs), 36 transfer RNAs (tRNAs), and 82 protein-coding genes (PCGs). The sequence comparison of two V. burejaeticum collected from Korea and China revealed 101 single nucleotide polymorphisms (SNPs) and 16 insertions/deletions (InDels). In addition, maximum-likelihood (ML) phylogenetic analysis indicated V. burejaeticum species collected in Korea and China are clustered together. This study provides useful information for future genetic study of V. burejaeticum.

Keywords: Adoxaceae; Viburnum burejaeticum; complete chloroplast genome; phylogenetic.

Figure 1.

Maximum likelihood tree based on the complete cp genome sequences of 10 species from the Adoxaceae family shows the 2 distinct clusters. Dipsacus japonicus used as outgroup. GenBank accession numbers are described in the figure. Shown next to the nodes are bootstrap support values based on 1000 replicates.