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Comment
. 2021 May 5;29(5):1664-1665.
doi: 10.1016/j.ymthe.2021.04.013. Epub 2021 Apr 22.

On your MARCKS, get set, deliver: Engineering extracellular vesicles

Kenneth W Witwer  1
Affiliations
Comment

On your MARCKS, get set, deliver: Engineering extracellular vesicles

Kenneth W Witwer. Mol Ther. .
No abstract available

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Figures

Figure 1
Figure 1
Identification and engineering of scaffold proteins Proteomic analysis identified two protein families that were enriched in stringently purified EVs: the EWI immunoglobulin superfamily (IGSF8 and PTGFRN) and the MARCKS protein family (MARCKS, MARKCSL1, and BASP1) (upper panel). The minimal sequences required for EV enrichment were delineated to serve as a scaffold protein via fusion to diverse payloads, including protein reporters, enzymes, antibody fragments, cytokines, tumor necrosis factor superfamily (TNFSF) ligands, RNA binding proteins, Cas9, and vaccine antigens (bottom panel).
See this image and copyright information in PMC

Comment on

  • A versatile platform for generating engineered extracellular vesicles with defined therapeutic properties.
    Dooley K, McConnell RE, Xu K, Lewis ND, Haupt S, Youniss MR, Martin S, Sia CL, McCoy C, Moniz RJ, Burenkova O, Sanchez-Salazar J, Jang SC, Choi B, Harrison RA, Houde D, Burzyn D, Leng C, Kirwin K, Ross NL, Finn JD, Gaidukov L, Economides KD, Estes S, Thornton JE, Kulman JD, Sathyanarayanan S, Williams DE. Dooley K, et al. Mol Ther. 2021 May 5;29(5):1729-1743. doi: 10.1016/j.ymthe.2021.01.020. Epub 2021 Jan 21. Mol Ther. 2021. PMID: 33484965 Free PMC article.

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