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. 2021 Oct;36(5):1073-1076.
doi: 10.1007/s12250-021-00384-w. Epub 2021 Apr 26.

Genetic Mutation of SARS-CoV-2 during Consecutive Passages in Permissive Cells

Ying Chen  1   2 , Mei-Qin Liu  1   2 , Yun Luo  1   2 , Ren-Di Jiang  1   2 , Hao-Rui Si  1   2 , Yan Zhu  1 , Bei Li  1 , Xu-Rui Shen  1   2 , Hao-Feng Lin  1   2 , Kai Zhao  1   2 , Ben Hu  1 , Zheng-Li Shi  1 , Xing-Lou Yang  3
Affiliations

Genetic Mutation of SARS-CoV-2 during Consecutive Passages in Permissive Cells

Ying Chen et al. Virol Sin. 2021 Oct.
No abstract available

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Susceptibility, replication efficiency, and mutations of SARS-CoV-2 in different cell lines. A Expression of N protein detected by immunofluorescence assays (IFAs) at 24 h post-infection (hpi) in SARS-CoV-2-infected cell lines, scale bar: 200 µm. B Viral loads detected by qRT-PCR in the culture supernatants of Calu-3, Caco-2, Huh-7, and Vero E6 cells infected with SARS-CoV-2 in duplicate at MOIs of 0.001, 0.01, 0.1, and 1. Supernatants were harvested at 0, 24, 48, 72, and 96 hpi. C Passage scheme and mutation information of SARS-CoV-2. D Schematic diagram of mutations in SARS-CoV-2 after isolated and passaged in Vero E6, Huh-7, and Caco-2 cell lines. The mutations were observed after the isolation (pink star), 2nd (blue triangle), 5th (red triangle or rectangle), and 6th (green triangle) passage.
See this image and copyright information in PMC

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