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Review
. 2021 Apr;37(4):379-385.
doi: 10.1051/medsci/2021034. Epub 2021 Apr 28.

[Cryo-electron microcopy for a new vision of the cell and its components]

[Article in French]
Daniel Lévy  1 , Aurélie Di Cicco  1 , Aurélie Bertin  1 , Manuela Dezi  1
Affiliations
Free article
Review

[Cryo-electron microcopy for a new vision of the cell and its components]

[Article in French]
Daniel Lévy et al. Med Sci (Paris). 2021 Apr.
Free article

Abstract
in English, French

Cryo-electron microscopy (cryo-EM) is a technique for imaging biological samples that plays a central role in structural biology, with high impact on research fields such as cell and developmental biology, bioinformatics, cell physics and applied mathematics. It allows the determination of structures of purified proteins within cells. This review describes the main recent advances in cryo-EM, illustrated by examples of proteins of biomedical interest, and the avenues for future development.

Title: La cryo-microscopie électronique révèle une nouvelle vision de la cellule et de ses composants.

Abstract: La cryo-microscopie électronique (cryo-EM) est une technique d’imagerie du vivant qui prend désormais une place prépondérante en biologie structurale, avec des retombées en biologie cellulaire et du développement, en bioinformatique, en biomédecine ou en physique de la cellule. Elle permet de déterminer des structures de protéines purifiées in vitro ou au sein des cellules. Cette revue décrit les principales avancées récentes de la cryo-EM, illustrées par des exemples d’élucidation de structures de protéines d’intérêt en biomédecine, et les pistes de développements futurs.

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References

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