Responses of Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) to Rice and Corn Plants, Fed and Oviposited by Spodoptera frugiperda (Lepidoptera: Noctuidae)

Abstract

The search behavior and parasitism of trichogrammatids can be affected by volatile compounds emitted by plants under herbivory and/or oviposition. Our aim was to evaluate the chemotactic behavior and parasitism rates of Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) females against two varieties of corn and one of rice that underwent herbivory or oviposition by Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae). In a glass Y-tube olfactometer, the parasitoids were given the choice between plants damaged by either herbivory or oviposition, with and without sentinel eggs, against those without damage. We also evaluated the average of parasitized eggs and the parasitoid emergence in sentinel eggs, which were next to plants that underwent herbivory contrasted with eggs next to undamaged plants. Trichogramma pretiosum was more attracted to rice and corn plants evaluated 24 h after herbivory compared to undamaged plants. Parasitoids preferred oviposited rice plants over control plants. Oviposited corn plants after 48 h were more attractive than non-oviposited plants without sentinel eggs. In the presence of sentinel eggs on the olfactometer tests, there was no difference in oviposition preference in corn. Parasitism was higher in sentinel eggs located near plants damaged by herbivory. This suggested that the egg parasitoid T. pretiosum not only uses chemical clues from rice and corn plants, damaged by herbivory, but also uses them as a strategy to search and increase parasitism in S. frugiperda eggs. However, the results of oviposition tests showed that plants of different species and varieties might respond differently to this type of damage.

Keywords: Parasitoid; fall armyworm; indirect defense; induced plant defense.