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. 2021 Apr 28;16(4):e0250852.
doi: 10.1371/journal.pone.0250852. eCollection 2021.

Antiplatelet activity and chemical analysis of leaf and fruit extracts from Aristotelia chilensis

Lyanne Rodríguez  1 , Andrés Trostchansky  2 , Irene Wood  2 , Mauricio Mastrogiovanni  2 , Hermine Vogel  3 , Benita González  3 , Mario Maróstica Junior  4   5 , Eduardo Fuentes  1 , Iván Palomo  1
Affiliations

Antiplatelet activity and chemical analysis of leaf and fruit extracts from Aristotelia chilensis

Lyanne Rodríguez et al. PLoS One. .

Abstract

Aristotelia chilensis (Mol.) Stuntz, also known as maqui, is a plant native to Chile without chemical characterization and quantification of the bioactive compounds present in it. HPLC-UV and HPLC-MS/MS studies have shown the presence, at different concentrations, of phenolic and anthocyanin compounds in fruit and leave extracts of the domesticated maqui clones Luna Nueva, Morena, and Perla Negra. The extracts from leaves and unripe fruits of Luna Nueva and Morena clones significantly inhibit platelet aggregation induced by several agonists; the extracts inhibit platelet granule secretion by decreasing the exposure of P-selectin and CD63 at the platelet membrane. Reactive oxygen species formation in platelets is lower in the presence of maqui extracts. Statistical Pearson analysis supports the levels of phenolic and anthocyanin compounds being responsible for the antiaggregant maqui effects. This work is the first evidence of antiplatelet activity from Aristotelia chilensis giving added value to the use of leaves and unripe fruits from this species.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Fig 1
Fig 1. Platelet aggregation induced by different agonists is modulated by maqui extracts.
Representative curves of platelet aggregation induced by ADP (A), collagen (B), TRAP-6 (C), and PMA (D). LNUF (EtOH/H2O): Ethanolic extract of Luna Nueva unripe fruit; MUF (H2O): Aqueous extract of Morena unripe fruit; MUF (EtOH/H2O): Ethanolic extract of Morena unripe fruit.
Fig 2
Fig 2. Effect of maqui extracts on platelet-granule release and ROS production.
The data shown correspond to the expression of P-selectin (A) and CD63 (B), and ROS production (C). Data are expressed as mean ± SEM, n = 3, from at least three independent experiments. * p<0.05, ** p<0.01 and *** p<0.001 vs. ADP (A and B) and antimycin A (C). MUF (H2O): Aqueous extract of Morena unripe fruit; MUF (EtOH/H2O): Ethanolic extract of Morena unripe fruit; LNUF (EtOH/H2O): Ethanolic extract of Luna Nueva unripe fruit.
Fig 3
Fig 3. Correlation between platelet aggregation inhibition and bioactive compounds levels in the extracts.
The correlation was established between the concentration of the compounds quantified in the most active maqui extracts and the % of platelet inhibition of these extracts. Data shown are on a 3-color scale: -1 blue indicates a maximum negative correlation; 1 red indicates a maximum positive correlation; white indicates a null correlation.
See this image and copyright information in PMC

References

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