Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 May-Jun;35(3):1461-1466.
doi: 10.21873/invivo.12398.

Differential Expression of BDNF and BIM in Streptozotocin-induced Diabetic Rat Retina After Fluoxetine Injection

Seong Taeck Kim  1 , Yoon Young Chung  2 , Hyo-In Hwang  2 , Hye-Kyoung Shin  2 , Ranju Choi  3 , Yong Hyun Jun  4
Affiliations

Differential Expression of BDNF and BIM in Streptozotocin-induced Diabetic Rat Retina After Fluoxetine Injection

Seong Taeck Kim et al. In Vivo. 2021 May-Jun.

Abstract

Background: Chronic diabetic retinopathy (DR) is a diabetic complication that causes blindness. Brain-derived neurotrophic factor (BDNF) expression is induced by fluoxetine. We observed the effects of fluoxetine on a streptozotocin (STZ)-induced diabetic rat model in this study.

Materials and methods: Rats were divided into three groups: Control, diabetic (65 mg/kg STZ injection), and diabetic with fluoxetine injection (20 mg/kg/week, six times). Western blotting was performed using anti-BDNF and anti-hexaribonucleotide-binding protein-3. Expression of BCL2 apoptosis regulator-like protein 11 (BIM) was analysed using a reverse transcription-polymerase chain reaction.

Results: BDNF levels were significantly higher in the diabetic group treated with fluoxetine than in the untreated diabetic group. BIM expression was higher in the diabetic group than in the control group. BIM gene expression was lower in fluoxetine-treated diabetic group than in the untreated diabetic group.

Conclusion: Fluoxetine had an anti-apoptotic effect with upregulation of BDNF expression in retina of rats with STZ-induced diabetes.

Keywords: BDNF; BIM; diabetic; fluoxetine; retina.

PubMed Disclaimer

Conflict of interest statement

The Authors declare no competing interests in regard to this study.

Figures

Figure 1
Figure 1. Timetable for the experimental procedures. Blood glucose levels were measured using the tail vein. A high glucose level (>250 mg/dl) was defined as diabetes mellitus (DM).
Figure 2
Figure 2. Analysis of serum glucose (A) and body weight (B) in rats of control, diabetes mellitus (DM) and fluoxetine-treated DM groups. The data are expressed as the mean±standard error of the mean. *Significantly different from the control at p<0.05.
Figure 3
Figure 3. Representative photographs (A) and quantification (B) of brain-derived neurotrophic factor (BDNF) expression on western blot in rats of control, diabetic (DM), and fluoxetine-treated DM groups. A: BDNF levels were significantly increased in the fluoxetine-treated group compared to that in the diabetic group. B: The results were expressed as a ratio compared to actin. The data are expressed as mean and standard error values. Significantly different at p<0.05 compared with *control and #DM.
Figure 4
Figure 4. The mRNA expression of BCL2 apoptosis regulator-like protein 11 (Bim) in retinas of normal, diabetes mellitus (DM) and fluoxetinetreated DM rats. The amounts of BIM mRNA expression were normalized to that of the internal control, glyceraldehyde 3-phosphate dehydrogenase. The data are expressed as mean and standard error values. Significantly different at: p<0.05 compared with *control and #DM.
See this image and copyright information in PMC

References

    1. Nazir MA, AlGhamdi L, AlKadi M, AlBeajan N, AlRashoudi L, AlHussan M. The burden of diabetes, its oral complications and their prevention and management. Open Access Maced J Med Sci. 2018;6(8):1545–1553. doi: 10.3889/oamjms.2018.294. - DOI - PMC - PubMed
    1. Guo X, Liu X. Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats. Mol Med Rep. 2017;16(2):2030–2036. doi: 10.3892/mmr.2017.6850. - DOI - PMC - PubMed
    1. Chen Q, Tan F, Wu Y, Zhuang X, Wu C, Zhou Y, Li Y, Cheng D, Wang J, Lu F, Shen M. Characteristics of retinal structural and microvascular alterations in early type 2 diabetic patients. Invest Ophthalmol Vis Sci. 2018;59(5):2110–2118. doi: 10.1167/iovs.17-23193. - DOI - PubMed
    1. Aldebasi YH, Rahmani AH, Khan AA, Aly SM. The effect of vascular endothelial growth factor in the progression of bladder cancer and diabetic retinopathy. Int J Clin Exp Med. 2013;6(4):239–251. - PMC - PubMed
    1. Toraih EA, Abdelghany AA, Abd El Fadeal NM, Al Ageeli E, Fawzy MS. Deciphering the role of circulating lncRNAs: RNCR2, NEAT2, CDKN2B-AS1, and PVT1 and the possible prediction of anti-VEGF treatment outcomes in diabetic retinopathy patients. Graefes Arch Clin Exp Ophthalmol. 2019;257(9):1897–1913. doi: 10.1007/s00417-019-04409-9. - DOI - PubMed

LinkOut - more resources