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Comment
. 2021 Apr 29;17(4):e1009547.
doi: 10.1371/journal.ppat.1009547. eCollection 2021 Apr.

Two courses of deconstructed coronavirus please

Connor G G Bamford  1
Affiliations
Comment

Two courses of deconstructed coronavirus please

Connor G G Bamford. PLoS Pathog. .
No abstract available

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Generation of “biologically contained” single-cycle SARS-CoV-2 viruses using virus reverse genetics and “trans-complementation”.
Wild-type virus and gene-deleted viruses are produced through construction and manipulation of full-length virus genomes. Wild-type virus can infect any susceptible cells, and outcoming virus is fully infectious. However, gene-deleted viruses (loss of Nsp3, E, or N) can only replicate in cells providing the essential deleted gene products “in trans.” Cells expressing the genes in trans will generally only be found within the lab, and not in somebody’s respiratory tract, making these systems much safer and capable to be used outside of a BSL3 lab. Created with BioRender.com. BSL3, Biosafety Level 3; SARS-CoV-2, Severe Acute Respiratory Syndrome Coronavirus 2.
See this image and copyright information in PMC

Comment on

  • A novel cell culture system modeling the SARS-CoV-2 life cycle.
    Ju X, Zhu Y, Wang Y, Li J, Zhang J, Gong M, Ren W, Li S, Zhong J, Zhang L, Zhang QC, Zhang R, Ding Q. Ju X, et al. PLoS Pathog. 2021 Mar 12;17(3):e1009439. doi: 10.1371/journal.ppat.1009439. eCollection 2021 Mar. PLoS Pathog. 2021. PMID: 33711082 Free PMC article.

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