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. 2021 Apr 4;26(7):2075.
doi: 10.3390/molecules26072075.

Bioactivity-Guided Isolation of Phytochemicals from Vaccinium dunalianum Wight and Their Antioxidant and Enzyme Inhibitory Activities

Tianrui Zhao  1 , Mengxue Sun  1 , Lingpeng Kong  1 , Qingwang Xue  2 , Yudan Wang  1 , Yifen Wang  3 , Afsar Khan  4 , Jianxin Cao  1 , Guiguang Cheng  1
Affiliations

Bioactivity-Guided Isolation of Phytochemicals from Vaccinium dunalianum Wight and Their Antioxidant and Enzyme Inhibitory Activities

Tianrui Zhao et al. Molecules. .

Abstract

Vaccinium dunalianum Wight, usually processed as a traditional folk tea beverage, is widely distributed in the southwest of China. The present study aimed to investigate the antioxidant, α-glucosidase and pancreatic lipase inhibitory activities of V.dunalianum extract and isolate the bioactive components. In this study, the crude extract (CE) from the buds of V. dunalianum was prepared by the ultrasound-assisted extraction method in 70% methanol and then purified with macroporous resin D101 to obtain the purified extract (PM). Five fractions (Fr. A-E) were further obtained by MPLC column (RP-C18). Bioactivity assays revealed that Fr. B with 40% methanol and Fr. D with 80% methanol had better antioxidant with 0.48 ± 0.03 and 0.62 ± 0.01 nM Trolox equivalent (TE)/mg extract for DPPH, 0.87 ± 0.02 and 1.58 ± 0.02 nM TE/mg extract for FRAP, 14.42 ± 0.41 and 19.25 ± 0.23 nM TE/mg extract for ABTS, and enzyme inhibitory effects with IC50 values of 95.21 ± 2.21 and 74.55 ± 3.85 for α-glucosidase, and 142.53 ± 11.45 and 128.76 ± 13.85 µg/mL for pancreatic lipase. Multivariate analysis indicated that the TPC and TFC were positively related to the antioxidant activities. Further phytochemical purification led to the isolation of ten compounds (1-10). 6-O-Caffeoylarbutin (7) showed significant inhibitory effects on α-glucosidase and pancreatic lipase enzymes with values of 38.38 ± 1.84 and 97.56 ± 7.53 µg/mL, and had the highest antioxidant capacity compared to the other compounds.

Keywords: Vaccinium dunalianum; antioxidant; enzyme inhibitory; guided isolation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structures of the isolated compounds (1–10).
Figure 2
Figure 2
Negative ion chemical profiles of CE, PM, and Fr. A–E from V. dunalianum buds. CE: crude extract, PM: purified extract by macroporous resin D101, and Fr. A–E are the five fractions.
Figure 3
Figure 3
Principal component analysis (PCA) biplot. Effect of CE, PM, and fractions from V. dunalianum buds on total phenolics, flavonoids, α-glucosidase, pancreatic lipase inhibitory effect, and antioxidant activity. Bar values with different letters are significantly different (p < 0.05). CE: crude extract, PM: purified extract, Fr. A–E mean five fractions.
Figure 4
Figure 4
Extraction and isolation procedure of compounds from the 70% methanol extract of V. dunalianum buds.
See this image and copyright information in PMC

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