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. 2021 Apr 8;18(8):3936.
doi: 10.3390/ijerph18083936.

Biocompatibility of a HA/β-TCP/C Scaffold as a Pulp-Capping Agent for Vital Pulp Treatment: An In Vivo Study in Rat Molars

Julia Guerrero-Gironés  1 , Antonia Alcaina-Lorente  2 , Clara Ortiz-Ruiz  3 , Eduardo Ortiz-Ruiz  4 , María P Pecci-Lloret  1 , Antonio José Ortiz-Ruiz  2 , Francisco Javier Rodríguez-Lozano  1 , Miguel R Pecci-Lloret  1
Affiliations

Biocompatibility of a HA/β-TCP/C Scaffold as a Pulp-Capping Agent for Vital Pulp Treatment: An In Vivo Study in Rat Molars

Julia Guerrero-Gironés et al. Int J Environ Res Public Health. .

Abstract

Bioceramic materials possess desirable biological properties, highlighting their non-reactivity and osteoconductivity. Their use has been extended in vital pulp treatment. The purpose of this study was to evaluate and compare the effects of beta-tricalcium phosphate (β-TCP), hydroxyapatite (HA), and collagen (C) scaffold with mineral trioxide aggregate (MTA) on the vital pulp of rat molars. Thirty-two molars of Sprague-Dawley rats underwent direct pulp capping with β-TCP/HA/C (n = 16) and MTA (n = 16). After 30 days, the following parameters were evaluated in the tested samples: the degree of pulp inflammation and pulp vitality, the presence of reparative dentin, the homogeneity of the odontoblastic layer, and the presence of pulp fibrosis. No statistically significant differences were observed between HA/β-TCP/C and MTA in terms of the degree of inflammation (p = 0.124). Significant differences were found in reparative dentin formation between the treatment groups (p = 0.0005). Dentin bridge formation was observed in the MTA-treated group. The local action of HA/β-TCP/C is similar to that of MTA when used as an agent for pulp vital treatment in terms of absence of inflammation and maintenance of pulp vitality, although there are significant differences between both materials regarding the formation of dentin bridges.

Keywords: beta-tricalcium phosphate; hydroxyapatite; mineral trioxide aggregate; scaffolds; vital pulp therapy.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Images of the pulp exposure site of two experimental groups at thirty days post-treatment. (a) MTA group. (b) HA/β-TCP/C group. Both groups showed a well-developed dentinal bridge (db) formed in contact with the material (asterisk) used for pulp capping. P = viable pulp without necrosis; D = dentin. Hematoxylin and eosin staining. Scale bar: 100 micrometers.
Figure 2
Figure 2
Images of a root canal at thirty days post-treatment. P = viable pulp without necrosis; D = dentin. (a) MTA group showed an intact odontoblastic layer (arrow) (b) HA/β-TCP/C showed fibrosis along the root canal. Masson’s trichrome staining. Scale bar: 100 micrometers.
See this image and copyright information in PMC

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