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. 2021 Apr 12;12(4):341.
doi: 10.3390/insects12040341.

Wing Morphometrics of Aedes Mosquitoes from North-Eastern France

Jean-Philippe Martinet  1   2 , Hubert Ferté  1   3 , Pacôme Sientzoff  1 , Eva Krupa  4 , Bruno Mathieu  4 , Jérôme Depaquit  1   3
Affiliations

Wing Morphometrics of Aedes Mosquitoes from North-Eastern France

Jean-Philippe Martinet et al. Insects. .

Abstract

Background: In the context of the increasing circulation of arboviruses, a simple, fast and reliable identification method for mosquitoes is needed. Geometric morphometrics have proven useful for mosquito classification and have been used around the world on known vectors such as Aedes albopictus. Morphometrics applied on French indigenous mosquitoes would prove useful in the case of autochthonous outbreaks of arboviral diseases.

Methods: We applied geometric morphometric analysis on six indigenous and invasive species of the Aedes genus in order to evaluate its efficiency for mosquito classification.

Results: Six species of Aedes mosquitoes (Ae. albopictus, Ae. cantans, Ae. cinereus, Ae. sticticus, Ae. japonicus and Ae. rusticus) were successfully differentiated with Canonical Variate Analysis of the Procrustes dataset of superimposed coordinates of 18 wing landmarks.

Conclusions: Geometric morphometrics are effective tools for the rapid, inexpensive and reliable classification of at least six species of the Aedes genus in France.

Keywords: Culicidae; geometric morphometrics; mosquito wings.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Sampling map.
Figure 2
Figure 2
Position of the 18 landmarks (recorded from the 1st to the 18th respectively) on an Aedes cinereus wing (scale bar = 500 µm).
Figure 3
Figure 3
Landmark positions after Procrustes superimposition.
Figure 4
Figure 4
Mean position of the 18 landmarks by mosquito species.
Figure 5
Figure 5
Canonical Variate Analysis of the Procrustes coordinates of Aedes mosquitoes.
Figure 6
Figure 6
Neighbor-joining tree performed over Mahalanobis distances and computed over 100 bootstrap replicates.
See this image and copyright information in PMC

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