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. 2021 Apr 25;14(9):2207.
doi: 10.3390/ma14092207.

Ceramic Biomaterial Pores Stereology Analysis by the Use of Microtomography

Żaneta Garczyk  1 , Zbigniew Jaegermann  2 , Piotr Duda  1 , Andrzej S Swinarew  1   3 , Sebastian Stach  1
Affiliations

Ceramic Biomaterial Pores Stereology Analysis by the Use of Microtomography

Żaneta Garczyk et al. Materials (Basel). .

Abstract

The main aim of this study was to analyze microtomographic data to determine the geometric dimensions of a ceramic porous material's internal structure. Samples of a porous corundum biomaterial were the research material. The samples were prepared by chemical foaming and were measured using an X-ray scanner. In the next stage, 3D images of the samples were generated and analyzed using Thermo Scientific Avizo software. The analysis enabled the isolation of individual pores. Then, the parameters characterizing the pore geometry and the porosity of the samples were calculated. The last part of the research consisted of verifying the developed method by comparing the obtained results with the parameters obtained from the microscopic examinations of the biomaterial. The comparison of the results confirmed the correctness of the developed method. The developed methodology can be used to analyze biomaterial samples to assess the geometric dimensions of biomaterial pores.

Keywords: alumina; image processing; microtomography apparatus; porous bioceramics.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Sample of a porous corundum biomaterial.
Figure 2
Figure 2
FT–IR ATR analysis of porous corundum biomaterial.
Figure 3
Figure 3
Microtomography image of corundum sample showing the porous surface of the biomaterial.
Figure 4
Figure 4
Measurement data for one of the samples—a 3D view and selected layer in the xy plane.
Figure 5
Figure 5
Area of analysis for one of the porous corundum biomaterial samples.
Figure 6
Figure 6
Microtomographic data analysis presented for one of the layers: (a) median filter; (b) binarization; (c) morphological opening; (d) disconnection of interconnected pores; (e) deletion of pores cut with the image frame; (f) result of the analysis; (g) result of the analysis—3D view.
Figure 7
Figure 7
Example of an image resulting from the analysis with SPIP software.
Figure 8
Figure 8
Distribution of equivalent diameter of the analyzed porous biomaterial samples.
Figure 9
Figure 9
Distribution of 3D area of the analyzed porous biomaterial samples.
Figure 10
Figure 10
Distribution of 3D volume of the analyzed porous biomaterial samples.
Figure 11
Figure 11
Distribution of pore diameters determined in images obtained using a confocal microscope.
See this image and copyright information in PMC

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References

    1. Mour M., Das D., Winkler T., Hoenig E., Mielke G., Morlock M.M., Schilling A.F. Advances in Porous Biomaterials for Dental and Orthopaedic Applications. Materials. 2010;3:2947–2974. doi: 10.3390/ma3052947. - DOI
    1. Szczepkowska M., Łuczuk M. Porous materials for the medical applications. Syst. Wspomagania Inżynierii Prod. 2014;2:231–239.
    1. Paciorek J. Materiały porowate—Właściwości i zastosowanie. Trendy ve vzdělávání. 2012;5:229–232.
    1. Perez R.A., Mestres G. Role of pore size and morphology inmusculo-skeletal tissue regeneration. Mater. Sci. Eng. C. 2016;61:922–939. doi: 10.1016/j.msec.2015.12.087. - DOI - PubMed
    1. Živić F., Grujović N., Mitrović S., Adamović D., Petrović V., Radovanović A., Durić S., Palića N. Friction and Adhesion in Porous Biomaterial Structure. Tribol. Ind. 2016;38:361–370.

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