Complete Genome Sequence of an Antimicrobial-Producing Bacillus velezensis Sam8H1 Isolate from the Makgadikgadi Saltpans of Botswana

Abstract

The global antimicrobial drug resistance crisis requires urgency in searching for more effective broad-spectrum antimicrobial drugs. Here, we present a complete circular genome sequence and a plasmid of an antimicrobial-producing isolate, Bacillus velezensis strain Sam8H1, from the Makgadikgadi saltpans in Botswana. Bioinformatic analyses revealed 12 putative secondary metabolite biosynthetic gene clusters important for genome-guided drug discovery studies.

FIG 1

Antimicrobial activity of the isolate. Zone of inhibition after growth on Luria broth (LB) (A) and activity on Bacillus cereus (B) and B. subtilis (C) when grown on tryptic soy agar (TSA). (D) Activity on E. coli when grown on LB; (E) activity on S. aureus when grown on nutrient agar (NA) supplemented with NaCl; (F) activity on Candida albicans after growth on LB supplemented with NaCl. (G) Concatenated ribosomal protein tree constructed using IQ-TREE. As shown, Bacillus velezensis Sam8H1 is closely related to B. amyloliquefaciens and B. velezensis with very good branch support. Maximum likelihood tree based on the LG+F+I+G4 model with branch support from SH-aLRT and UF-boot.