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. 2021:2285:99-109.
doi: 10.1007/978-1-0716-1311-5_8.

TCR Vβ Evaluation by Flow Cytometry

Benedetta Peruzzi  1 , Sara Bencini  1 , Roberto Caporale  2
Affiliations

TCR Vβ Evaluation by Flow Cytometry

Benedetta Peruzzi et al. Methods Mol Biol. 2021.

Abstract

T-cell receptor (TCR)-Vβ repertoire analysis is a sensitive method for detection of T-cell clonality. This type of analysis has been used for studying selective T-cell responses in autoimmune disease, alloreactivity in transplantation, and protective immunity against microbial and tumor antigens and in neoplastic T cells. Here, we describe the flow cytometric methods to perform this analysis.

Keywords: Clonality; Clonogram; Flow cytometry; Monoclonal antibodies; Repertoire; T-cell receptor (TCR)-Vβ.

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